液相沉积法制备纳米二氧化钛涂层毛细管及其在蛋白质和多肽分离中的应用
首发时间:2009-01-14
摘要:毛细管电泳中,由于酸性及中性条件下蛋白质易与管壁发生吸附;碱性条件下,淌度差异小的蛋白质会很快被较高的电渗流驱出毛细管,得不到充分分离。因而未改性的熔融石英毛细管不适于直接分离蛋白质及多肽。为了解决这些问题,本文用液相沉积法制备了纳米二氧化钛涂层毛细管,通过X射线光电子能谱初步研究了纳米二氧化钛涂层的化学性质,考察了磷酸盐缓冲液中该管的电渗流性质,并将其应用于蛋白质和多肽的开管毛细管电色谱分离。
关键词: 液相沉积 二氧化钛 开管毛细管电色谱 蛋白质多肽分离
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Open Tubular Capillary Electrochromatographic Separation of Proteins and Peptides Using a TiO2 Nanoparticle-Deposited Capillary by Liquid Phase Deposition
Abstract:In capillary electrophoresis, fused-silica capillary is not appropriate for the direct separation of proteins and peptides without any modification on the inner surface. Because at acidic/neutral pH, strong interaction would occur between the deprotonated silanol groups and the basic parts of biomolecules; while at basic pH, analytes with small differences in mobility would be quickly ejected through the capillary by a high EOF without sufficient resolution. In order to overcome these shortcomings, we prepared a titanium dioxide (TiO2) nanoparticles (NP)-deposited capillary by liquid phase deposition (LPD) method for open tubular capillary electrochromatographic (OTCEC) separation of proteins and peptides. Its surface chemical property was studied by X-ray photoelectron spectroscopy (XPS) experiment. Its electroosmotic properties and separation mechanism in phosphate buffers were investigated as well.
Keywords: liquid phase deposition titanium dioxide open tubular capillary electrochromatography protein and peptide separation
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液相沉积法制备纳米二氧化钛涂层毛细管及其在蛋白质和多肽分离中的应用
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