改善酶固定以增强葡萄糖传感器的生物电化学活性
首发时间:2009-01-15
摘要:在本工作中,我们研发了一种基于多壁碳纳米管和氧化锌纳米粒子协同作用的高灵敏度和高稳定性的葡萄糖传感器。因为葡萄糖氧化酶与氧化锌的等电点存在明显的差异,所以葡萄糖氧化酶与氧化锌能牢固静电键合,形成独特的多层结构:PDDA/GOx/ZnO/MWNTs,为固定化酶提供了良好的微环境,既保持了酶的生物活性,又避免了酶的泄漏。载有0.5单位GOx的传感器其线性范围为0.1至16 mM;载有2.0单位GOx的传感器其灵敏度为50.2 mA cm-2M-1,检测极限为250 nM,长期稳定性为160天。100个人血样的测定结果与传统的分光光度法的测定结果符合(相关系数为0.997)。
关键词: 葡萄糖传感器 葡萄糖氧化酶 多壁碳纳米管 氧化锌纳米粒子 协同作用
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Improved enzyme immobilization for enhanced bioelectrocatalytic activity of Glucose sensor
Abstract:In this work, we report the development of a highly sensitive and stable glucose sensor based on the synergetic effect of multi-walled carbon nanotubes (MWNTs) and ZnO nanoparticles. Since the isoelectric point (IEP) of glucose oxidase (GOx) is significantly different from that of ZnO nanoparticles, GOx was electrostatically bound to ZnO nanoparticles decorated onto a negatively charged MWNTs layer at the electrode surface via VLS growth. A cationic polydiallyldimethylammonium chloride (PDDA) layer was further coated onto the GOx-contained ZnO nanoparticle layer to prevent the leakage of GOx. This unique multi-layer structure (PDDA/GOx/ZnO/MWNTs) provided a favorable microenvironment to maintain the bioactivity of GOx, which led to rapid amperometric response toward glucose. By loading 0.5-unit Gox at the sensor surface, we obtained a wide linear response range of 0.1 to 16 mM for this sensor. High sensitivity of 50.2 mA cm-2M-1 was obtained with increased loading of GOx (2.0 units), leading to an even lower detection limit of 250 nM. This nanomaterials-based glucose sensor was highly sensitive and showed favourable stability over a relatively long-term storage (160 days). Importantly, we challenged our sensor with 100 human blood serum samples, and obtained consistent results with the classic spectrometric assay (correlation coefficient, 0.997).
Keywords: Glucose sensorptt Glucose oxidase MWNTs ZnO particles synergic action
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No.2792111882512320****
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