PC12 cells injured by Amyloid –protein and effected by the Calciumion internal flowing by cerebrospinal fluid with drug-containing Dihuangyinzi.

• 1、Heilongjiang University of Chinese Medicine

Abstract：Discuss the cerebrospinal fluid with ancient prescriptions Dihuangyinzi which has protective function on PC12 cells, injured by β-amyloid protein (A β25-35). Method: We divide PC12 cells into 6 groups—that is, the blank group, the model group, the control group of western medicine, and the cerebrospinal fluid groups with low-dose, medium-dose and high-dose of Dihuangyinzi with pharmacology method of cerebrospinal fluid containing Chinese medicine; and add normal culture medium, the normal cerebrospinal fluid and cerebrospinal fluid containing drugs respectively；after incubation, then add calcium fluorescent agent flou-3/AM and observe the content of calcium in the cell under the laser scanning microscope after load。Results: Although fluorescence intensity in each cerebrospinal fluid group increases after adding A β25-35, the average strength of the fluorescence intensity reaching its peak decreases significantly when compares with the model group; the difference has statistics significance (P <0.01), and the fluorescence intensity of medium and high groups decreases when compares with western medicine control group，and the difference has statistics significance (P <0.05). Conclusion: Cerebrospinal fluid with Dihuangyinzi can reduce Calciumion, induced by Aβ, internal flowing.

Keywords： Dihuangyinz Alzheimei\