Effect of tetramethylpyrazine on spatial cognition and cell proliferation of SVZ after focal cerebral ischemia in rat

• 1、Institute of neurobiology,Xi’an Jiaotong University School of medicine

Abstract：Object: In the study, we aim to demonstrate the effect of tetramethylpyrazine (TMP) on spatial learning and memory, and if TMP can induce cell proliferation of SVZ after focal cerebral ischemia in rat. Methods: The focal cerebral ischemic model of rat was made by suture occlusing the middle cerebral artery. TMP was administered introperitoneally after MCAO. The spatial learning and memory function was studied by Morris water maze test. The cell proliferation of SVZ was labelled with BrdU and BrdU positive cells were showed by Immunohistochemical stain. Results: The ischemia group exhibited seriously spatial learning and memory deficits. There was a significant increase of BrdU positive cells in SVZ after MCAO. In place navigation trial, the mean values of escape latency of TMP group were shorter than those of ischemia group (P<0.05). In sp atial probe trial , there we re significant difference in the time percentage of spending in the former platform quadrant and frequency of crossing the former platform site between TMP group and ischemia group (P < 0. 05) . There was a significant increase of BrdU positive cells in SVZ of TMP groupcompared to ischemia group (p<0.05) at 3d and 7d. Conclusion: TMP can improve the spatial congnition defects induced by the focal cerebral ischemia, and promote cell proliferation in SVZ.

Keywords： Tetramethylpyrazine；Learning and memory；SVZ；Cell Proliferation