Ultrasonic Vibration Microdissection technology

• 1、Robotics Institute , Harbin Institute of Technology.

Abstract：Molecular techniques are transforming our understanding of cellular function and the origin of disease. In order to get the cells or other tissues precisely, many methods have been used. One of them is ultrasonic vibration microdissection (UVM). In our laboratory, an ultrasonic vibration microdissection System for molecular analysis of tissue had been developed in the last 2years. In this system, a multilayer piezoelectric actuator is used to actuate a sharp microneedle vibrating with high frequency and low amplitude to cut the tissue. Here, we focus on the principle and parameters setup for the ultrasonic vibration microdissection. Firstly, the ultrasonic vibration microdissection process was analyzed, and then the parameters effects for microdissection were put forward. It is helpful for setting the parameters, thus as vibration amplitude and frequency, properly to get the ideal cutting effect. Finally, contrast experiments were carried out, and the experiment results show agreement with the analyses results. Also, a circle track cutting performance was shown, form which it can be seen that that the embedded tissue can be quickly and precisely cut with the ultrasonic vibration microdissection method under the proper parameters

Keywords： microdissection multilayer piezoelectric actuator ultrasonic vibration