天山雪莲悬浮细胞培养物中紫丁香苷的分离及含量测定
首发时间:2009-03-27
摘要:紫丁香苷为天山雪莲的主要活性成分之一,本研究运用溶剂提取方法,结合各种色谱手段对天山雪莲组织细胞培养物中的紫丁香苷进行分离纯化,并根据现代谱学方法对其结构进行鉴定。同时采用高效液相色谱法建立了天山雪莲组织细胞培养物中紫丁香苷的含量测定方法,该方法简便,精密度、重现性良好,结果准确可靠。这些研究为通过天山雪莲细胞悬浮培养大规模生产活性成分紫丁香苷奠定了基础。
For information in English, please click here
Isolation and Analysis of Syringin in Cell Suspension Cultures of Saussurea involucrata
Abstract:Syringin is one of the main bioactive ingredients in Saussurea involucrata. In this study, various chromatographic techniques were employed to isolate and purify syringin in the polar extraction of cell suspension cultures of S. involucrata. The structure of syringin was characterized by the analysis of spectroscopic data. A quantitative analytical method for the content of syringin in cultures of S. involucrata was established by RP-HPLC. The method is convenient, accurate and reliable. All this results provided a basis for further studies on application of cell suspension cultures of S. involucrata for large-scale production of bioactive compound syringin.
Keywords: Saussurea involucrata syringin cell suspension cultures RP-HPLC
论文图表:
引用
No.3085245362312381****
同行评议
共计0人参与
勘误表
天山雪莲悬浮细胞培养物中紫丁香苷的分离及含量测定
评论
全部评论0/1000