Effect of sonodynamic therapy on singlet oxygen content of C6 glioma cells of SD rat after ultrasound radiation

• 1、 The Fourth Affiliated Hospital of Harbin Medical University

Abstract：Objective:Content of C6 glioma cells of SD rat after ultrasound radiation was to be studied in this paper. Materials and Methods: Subcutaneous injection of C6 glioma cells was performed for 56 SD rats, after tumor reached 2 cm, these rats were randomly divided into 7 groups, 8 rats/group, among which one group was taken as control group, and sonosensitizer hematoporphyrin monomethyl ether (HMME, 10mg/kg) was injected into tumor with control group as exception. After injection, these rats were treated by sonotodynamic therapy with 120s ultrasound radiation of 0.5W/cm2 power and 1 MHz frequency, 0h, 6h, 12h, 24h, 48 and 72h after radiation, tumor samples were collected, frozen and storied in liquid nitrogen, and dried by vacuum freeze, their Singlet oxygen contents were determined by Electron Paramagnetic Resonance Spectrometer after being ground. Results: At selected dosage, single oxygen concentration increased gradually after irradiation and reached peak at 24 h and decreased, and single oxygen content decreased significantly after 48 h of irritation..Conclusion: One inhibitory path of sonotodynamic therapy for tumor is that yield of Singlet oxygen was improved rapidly in short time after sonotodynamic therapy, which had DNA soundsensitive effect on tumor, acted on cell membrane, mitochondria and nucleic acid and reached the aim of killing tumor cells.

Keywords： sonotodynamic therapy C6 glioma HMME single oxygen