涤痰汤对AD模型大鼠行为学影响的实验研究
首发时间:2009-04-03
摘要:目的:观察涤痰汤对阿尔茨海默病(AD)模型大鼠行为学的影响。方法:将大鼠随机分为正常组、模型组、假手术组、涤痰汤组和哈伯因组,采用大鼠前脑基底核注射Aβ25-35和IBO(鹅膏覃氨酸)混合液诱导复合AD动物模型,涤痰汤组和哈伯因组灌胃给药28天后,运用Morris水迷宫系统测定正常组、模型组、假手术组、涤痰汤组、哈伯因组的逃避潜伏期及跨台次数。结果:AD模型组大鼠空间学习记忆能力(逃避潜伏期、平均游泳距离、跨台次数)与正常组、哈伯因组、涤痰汤组分别比较均有显著统计学差异。而在定位航行试验中,涤痰汤组前2天逃避潜伏期、40%区域较哈伯因组明显减少,有显著统计学差异;在空间搜索试验中,涤痰汤组20%区域少于哈伯因组,差异显著。其余指标两组间均无明显差异。正常组与假手术组相比各项指标均无显著差异。结论:涤痰汤对于采用大鼠前脑基底核注射IBO和 Aβ25-35混合液诱导造成AD模型大鼠的空间学习记忆功能受损具有明显保护作用,且涤痰汤的保护作用稍优于西药哈伯因。
关键词: 阿尔茨海默病 涤痰汤 Morris水迷宫系统 逃避潜伏期 穿越原平台次数
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The empirical study of the influence of Ditan decoction on the behavior on rat model with Alzheimer’s disease
Abstract:Objective: To observe the Influence of Ditan Decoction on the Behavior on Rat Model with Alzheimer’s Disease.Methods:All the rats were randomly divided into five groups: normal group,control group,sham operated group,Ditan decoction group and Huperzine A-Zhulin Antun treated group,then induced by injection of basal nucleus of Meynert(BNM)with the composition of ibotenic(IBO) and Aβ25-35 t o make AD model. After treating for 4 weeks with Ditan decoction group and Huperzine A-Zhulin Antun treated group, Morris water maze was used to assay the ability of escaping incubation period and the frequency of jumping table of the five groups.Results: The learning and memory ability of the model rats have significant difference with normal group, Ditan decoction group and Huperzine A-Zhulin Antun treated group.In behavior experiment,averge incubation period of the Ditan decoction group have obvious shorten compared to the Huperzine A-Zhulin Antun treated group. In space research,the frequency in span of platform localization of the Ditan decoction group have obvious shorten compared to the the Huperzine A-Zhulin Antun treated group.The other index of the two group have no difference.The normal group and sham operated group have no difference.Conclusion: Ditan decoction have obvious protection of the learning and memory ability of the Rat Model with Alzheimer’s Disease induced by injection of basal nucleus of Meynert(BNM)with the composition of ibotenic(IBO) and Aβ25-35.And the protection of Ditan decoction is better than Huperzine A-Zhulin Antun.
Keywords: Alzheimer’s disease Ditan decoction Morris water maze escaping incubation period frequency of jumping table
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