MALDI-TOF-MS Analysis of Small Molecules Using Modified Mesoporous Material SBA-15 as Assisted Matrix
首发时间:2009-04-06
Abstract:Mesoporous silica, SBA-15 has been successfully functionalized with quinoline moiety and applied to MALDI-TOF-MS analysis of small molecules as a matrix. The modified SBA-15 material (SBA-15-8-(3-(triethoxysilyl)propoxy)quinoline, SBA-15-8QSi) were obtained by using calcined SBA-15 and 8-hydroxyl quinoline. The structure of the functionalized mesoporous was systemically characterized with TEM technique, N2 adsorption-desorption isotherms technique and FT-IR spectra. Compared with DHB and SBA-15, it is demonstrated several advantages in the analysis of low molecules with MALDI-TOF-MS, such as less background interference ions, high homogeneous, better reproducibility etc. Base on these results, the various kinds of effect parameters were optimized. The ideal operation conditions are 1) methanol used as dissolving solvent; 2) sample first dropping method; 3) ratio between the analyte and matrix is 3.5:10. Under the optimization conditions, it gives low detection limit (0.1 fg for sucrose) and high reproducibility (≤29%). And the technique was successfully applied in analysis of low molecular saccharides and natural honey.
keywords: Matrix MALDI-TOF-MS low molecules honey SBA-15
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MALDI-TOF-MS分析小分子用修饰的介孔材料SBA-15作为基质
摘要:本论文中成功的将喹啉修饰到介孔材料SBA-15上,并且将其作为基质用于MALDI-TOF-MS中分析小分子。修饰化的SBA-15(SBA-15-8-(3-三甲氧基硅烷基丙基喹啉,即SBA-15-8QSi)通过SBA-15和8-(3-三甲氧基硅烷基丙基)喹啉反应制得。修饰后的介孔材料的结构系统的用TEM技术,N2吸附-脱附等温技术和红外光谱表征。在MALDI-TOF-MS分析低分子量分子时与DHB相比,其具有许多有点,例如:较少的背景干扰离子峰、高的均一性、更好的重现性等等。基于以上的这些结论,优化了多方面的影响参数。优化的测试条件:甲醇做为溶解溶剂;先点样品的点样方法;分析物和基质的比例是3.5:10。在优化的条件下得到了最低检测限(蔗糖:0.1fg)和高的重现性(≤29%)。这一技术成功的应用到分析低分子量的糖类化合物和天然蜂蜜中。
关键词: 基质 MALDI-TOF-MS 低分子量分子 蜂蜜 SBA-15
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No.3108645473912389****
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