胡黄连苦苷Ⅱ在大鼠体内药动学研究及其血浆蛋白结合率的测定
首发时间:2009-04-10
摘要:目的:建立大鼠血浆中胡黄连苦苷Ⅱ的HPLC-UV测定方法,研究在大鼠体内的药代动力学特征,同时测定其血浆蛋白结合率。方法:血浆样品经简单的甲醇沉淀蛋白后,上清液直接进样测定。采用Diamonsil®(钻石)C18色谱柱(4.6 mm×250 mm,5μm)流动相为甲醇-水-醋酸(38:62:0.2),检测波长267 nm,流速1 ml/min,采用3p97药动学软件对药时数据进行拟合。结果:大鼠尾静脉注射胡黄连苦苷Ⅱ符合二室开放模型。结论:胡黄连苦苷Ⅱ在大鼠体内分布代谢很快,消除较快。
For information in English, please click here
Studies on pharmacokinetics of picrosideⅡ in rat in vivo and detection of its plasma protein binding rate
Abstract:Objective:To esteblish a sensitive HPLC method with UV detection for the determination of picrosideⅡin rat plasma and to study the characteristic of Pharmacokinetics in rat in vivo, detection of its plasma protein binding. Method: The supernatant liquid was determined after the different plasma samples proteins were precipitated by methanol. Diamonsil®C18 column (4.6 mm×250 mm,5 μm)was used with a mobile phase of CH3OH:H2O:CH3COOH(38:62:0.2). The detection wavelength was 267nm, the flow rate was 1 ml•min-1. The plasma drug concentration-time data was calculated by 3p97 pharmacokinetics software. Results: It was fitted two-compartment model after inject picrosideⅡ to rat vena caudalis. PicrosideⅡdistributed and metabolized quickly, eliminated soon in rat in vivo. Conclusion: This HPLC method was accurate, simple, sensitive and provide reference in detecting blood concentration of picrosideⅡ.
Keywords: pcrosideⅡ pharmacokinetics plasma protein binding rate
论文图表:
引用
No.3126610942612393****
同行评议
共计0人参与
勘误表
胡黄连苦苷Ⅱ在大鼠体内药动学研究及其血浆蛋白结合率的测定
评论
全部评论0/1000