HPLC同时测定生脉饮中人参皂苷Rb1、Rg1的含量
首发时间:2009-04-23
摘要:目的:用高效液相色谱法同时测定生脉饮中人参皂苷Rb1、Rg1的含量。方法:采用Diamonsil C18柱(5 μm,250 mm×4.6 mm),乙腈-水进行梯度洗脱,流速0.6ml•min-1,检测波长203nm,柱温25℃。结果:对生脉饮中人参皂苷Rb1和人参皂苷Rg1进行含量测定。其线性范围分别为0.050~1.000mg ml-1(r=0.9994)和0.050~1.000mg ml-1(r=0.9991),平均回收率分别为98.3%(RSD=4.3%)和96.5%(RSD=2.1%)。结论:方法简便,可用于生脉饮质量的评价。
关键词: 高效液相色谱 生脉饮 人参皂苷Rb1 人参皂苷Rg1
For information in English, please click here
Simultaneous Determination of Ginsenoside Rb1 and Ginsenoside Rg1 in Shengmaiyin by HPLC
Abstract:Objective:To determine the content of ginsenoside Rb1 and ginsenoside Rg1 in shengmaiyin simultaneously by HPLC .Methods: The quantitative assess was carried out on a Diamonsil C18 column(5 μm,250 mm×4.6 mm),with a moble phase of acetonitrile-water at a flow rate of 0.6 ml•min-1,and UV detection was performed under 203nm with 25℃ as the column temperature .Result: Quantitative assess was performed on ginsenoside Rb1 and ginsenoside Rg1. The linear ranges for ginsenoside Rg1 and ginsenoside Rb1 were 0.050-1.000mg•ml-1(r=0.9994) and 0.050-1.000mg•ml-1 (r=0.9991) , respectively . The average recoveries were 98.3(RSD=4.3%)and 96.5%(RSD=2.1%), respectively.Conclusion:The method is simple,and it can be used to evaluate the quality of shengmaiyin.
Keywords: HPLC shengmaiyin ginsenoside Rb1 ginsenoside Rg1
基金:
论文图表:
引用
No.3166446122512404****
同行评议
共计0人参与
勘误表
HPLC同时测定生脉饮中人参皂苷Rb1、Rg1的含量
评论
全部评论0/1000