反相高效液相色谱分离16种多环芳烃
首发时间:2009-06-24
摘要:采用反相高效液相色谱-紫外检测器-荧光检测器(RP-HPLC-UVD-FLD)法,对16种多环芳烃进行分离和检测,对流动相、流速、荧光吸收波长等条件进行优化。结果表明,除苯并(a)蒽和 、二苯并(a,h)蒽和苯并[g,h,i]苝不能分开外,其它组分都能实现较好分离。该方法紫外检测保留时间的相对标准偏差在0.48%~2.93%,响应值的相对标准偏差在0.59%~7.72%;荧光检测保留时间的相对标准偏差在0.09%~3.66%,响应值的相对标准偏差在0.39%~7.68%。多环芳烃的紫外检测器最低检测限为0.0193 ng~0.7739 ng;荧光检测器最低检测限为0.0015ng~2.0179 ng。
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Separation and Determination of Sixteen PAHs by Reverse–phase High Performance Liquid Chromatography
Abstract:Reverse–phase high performance liquid chromatography with connection of ultraviolet detector and fluorescent detector in series (HPLC-UVD-FLD) was adopted to analyze and separate 16 PAHs. The analysis conditions were optimized for mobile phase, the flow rate and fluorescent wavelength. The results showed that 16 PAHs were better separated off except benzo(a)anthracene and chrysene, dibenzo(a,b)anthracene and benzo(g,h,i)perylene. The RSD of retain time and relative response factors of HPLC-UVD ranged from 0.48% to 2.93% and 0.59% to 7.72%, and the RSD of retain time and relative response factors of HPLC-FLD ranged from 0.09% to 3.66% and 0.39% to 7.68%, and the limits of detection of 16 PAHs of HPLC-UVD and HPLC-FLD ranged from 0.0193ng to 0.7739ng and 0.0015ng to 2.0179ng, respectively.
Keywords: PAHs RP-HPLC separation determination
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