石油降解菌的诱变与降解性能研究
首发时间:2009-06-30
摘要:随着石油工业的发展,石油对环境的污染将会日趋严重。已有的研究结果和应用实践表明微生物治理石油污染的技术是可行的。本实验筛选出降解石油性能较好的菌株,并通过诱变育种选择高效石油降解菌,研究该菌株对石油的降解效果以及诱变前后环境条件对石油降解率的对比,得出结论:诱变时间为40s时的菌株去除石油的效果最好。诱变菌对石油降解规律与初始菌对石油的降解趋势相似,诱变菌接种量越多,石油的降解效果越好;石油去除率随着初始石油浓度的增加而降低;微生物对石油的降解经过调整期,旺盛期,稳定期三个阶段。不同的是,诱变菌在初始pH值为8时,石油去除率最佳。
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Study on the Induced Mutation and Function of Degrading Petroleum Baceria
Abstract:With the development of the petroleum industry, oil pollution will become more serious. Informed practice and application of research results indicate that microbial treatment of oil pollution technology is feasible. In this study, we separated and domesticated bacteria with obvious oil degradation ability,then singled out the highest effect degrading bacteria via UV light irradiate breeding. Through the study of the strain on oil degradation as well as the effect of environmental conditions before and after the mutation of the oil degradation rate of contrast, we got the conclusions as follows: Mutation 40 seconds,the strain was the most effective removal of oil. Strain induced degradation of the oil law and the original strain on oil degradation trends similar to those induced bacteria inoculum more oil, the more doses were added, the more efficient degradation rate was; While the density of oil increased, the rate of oil degradation reduced;microbial oil After the degradation of an adjustment period, the period of strong and stable period of three stages. The difference is that when the pH value was 8, the mutation bacteria strain had the best oil degradation rate.
Keywords: Petroleum Biodegradation UV light irradiate Degrading bacteria Degradation rate
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No.3351145098012463****
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