高效溶菌酶脂质体的制备及其对生物膜的剥离研究
首发时间:2009-07-07
摘要:本文旨在制备环境友好型管道生物膜剥离剂,并综合运用物化和生化手段评价其作用效果。实验采用逆向蒸发法制备了稳定的超细溶菌酶脂质体,平均粒径为80~100nm,包封率为82.4%。Zeta电位、TEM和SEM结果表明脂质体在溶液中分散均匀。在不锈钢表面培养出稳定生物膜后,分别利用溶菌酶和溶菌酶脂质体对其进行剥离。溶菌酶脂质体对混合菌种形成的稳定生物膜剥离效率达到86.5%,相同浓度下溶菌酶剥离效率为62.4%。SEM和FT-IR表征结果亦证明了溶菌酶及其脂质体具有良好的生物膜剥离效率。溶菌酶脂质体对生物膜和水体中微生物去除分别达到一个和三个级数,能够有效控制管道中的生物膜和微生物风险。
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Lysozyme-liposome synthesis and its capability on biofilm removal
Abstract:This paper aims to prepare environment-friendly reagents for biofilm removal and utilize physiochemical and biochemical methods to assess their capability. Super-stable ultra-fine lysozyme-liposomes with an average diameter of 80~100 nm were synthesized via reverse-phase evaporation (VET). Zeta potential, TEM and SEM analysis showed the liposomes uniformly dispersed in the solution. Lysozyme and its liposomes were separately used to remove the stable biofilms formed on the stainless steel surface. Under the same concentration condition, lysozyme-liposomes could effectively remove total biomass of the biofilm up to 86.5 %, while free lysozyme could remove 62.4 %. SEM morphology and FT-IR spectroscopy of biofilms also provided solid evidence of biomass removal by lysozyme and its liposomes. In addition, lysozyme-liposome could reduce bacterial in biofilm and solutions by 1-log and 3-log, respectively, in 24 hours, which greatly eliminates microorganism risks in pipeline.
Keywords: liposome lysozyme biofilm removal mixed bacterial consortium
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