基于多壁碳纳米管-金纳米复合物的乙酰胆碱酯酶生物传感器研制
首发时间:2009-09-16
摘要:一步合成了多壁碳纳米管-金纳米复合物(MWCNTs-Au)。该复合物具有极好的亲水性,有利于生物分子的吸附。本文将乙酰胆碱酯酶固定在MWCNTs-Au纳米复合物修饰的玻碳电极上构建了新型的电流型乙酰胆碱酯酶生物传感器。由于纳米复合界面良好的导电性,固定的乙酰胆碱酯酶对其底物硫代乙酰胆碱显示出极高的亲和性。有机磷农药能强烈抑制胆碱酯酶活性,抑制率的变化为监测有机磷农药提供了新途径。以马拉硫磷为有机磷农药模型分子,乙酰胆碱酶的抑制率与马拉硫磷浓度在1.0-1000 ng mL-1 和2-15 μg mL-1 之间呈现良好的线性关系,检测限0.6 ng mL-1。该传感器重现性好,稳定性高、检测速度快,检出限低,为测定胆碱酯酶的抑制剂提供了新方法。
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multiwalled carbon nanotubes, nanocomposites, acetylcholinesterase, malathion
Abstract:A facile, one step synthesis of nanocomposites using multiwalled carbon nanotube coating gold nanoparticles (MWCNTs-Au) was presented. The formed MWCNTs-Au nanocomposites offered an extremely hydrophilic surface for biomolecule adhesion, leading to a stable acetylcholinesterase (AChE) biosensor. Due to the excellent conductivity of the nanocomposites, the immobilized AChE showed favorable affinity to acetylthiocholine (ATCl) and could catalyze the hydrolysis of ATCl with a Kmapp value of 268 μM to form thiocholine, which was then oxidized to produce a detectable and fast response. Based on the inhibition of organophosphates (OPs) on the enzymatic activity of AChE, the magnitude of peak current from thiocholine on the biosensor is a simple and effective way to biomonitoring of OPs exposure. Using malathion as a model compound, the inhibition of malathion was proportional to its concentration ranging from 1.0 to 1000 ng mL-1 and from 2 to 15 μg mL-1, with a detection limit 0.6 ng mL-1. The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors.
Keywords: multiwalled carbon nanotubes nanocomposites acetylcholinesterase malathion
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基于多壁碳纳米管-金纳米复合物的乙酰胆碱酯酶生物传感器研制
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