添加胰蛋白酶促进Streptomyces hygroscopicus产谷氨酰胺转胺酶
首发时间:2009-09-22
摘要:研究了添加胰蛋白酶对Streptomyces hygroscopicus合成谷氨酰胺转胺酶的影响。结果表明,添加胰蛋白酶可以提高发酵过程中谷氨酰胺转胺酶的酶活。摇瓶培养中,在发酵起始时添加200U/mL的胰蛋白酶,谷氨酰胺转胺酶的酶活最高达到了6.61U/mL,比对照提高了27.1%。初步研究表明,添加胰蛋白酶可以直接切割发酵过程中产生的酶原,使其被快速地转化为成熟酶,解除了酶原的产物抑制作用,产生更多的酶原,从而促进了产酶。
关键词: 谷氨酰胺转胺酶 Streptomyces hygroscopicus 胰蛋白酶 酶原
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Enhancement of Microbial Transglutaminase by Streptomyces hygroscopicus Using Tripsin
Abstract:Effect of Tripsin addition on the accumulation of microbial transglutaminase(MTG) by Streptomyces hygroscopicus was investigated. The results showed that the addition of Trypsin enhanced MTG accumulation, and the optimal addition time and concentration of Trypsin were at 0 h and 200 U/mL. The maximum MTG activity in the culture broth was 6.61 U/mL and increased by 27.1% compared with the control. With the addition of Trypsin, pro-TGase was cut to MTG, thus adding Trypsin into the culture broth could enhance the production of pro-TGase by relieving the product inhibition, and the accumulation of MTG was improved.
Keywords: Microbial Transglutaminase Streptomyces hygroscopicus Trypsin pro-MTG
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