荧光相图法分析温度对溶菌酶变性中间体的影响
首发时间:2009-11-13
摘要:本文应用荧光相图讨论了温度对法溶菌酶的变性中间体的影响并对溶菌酶变性模型进行了讨论。在4摄氏度,尿素浓度为5摩尔/升时,溶菌酶变性中间体在荧光相图中清晰可见,变性过程符合三态模型。但是在25和37摄氏度时,由于温度对影响,溶菌酶由天然态到变性中间体和变性有中间体到变性态两过程的反应常数不同,溶菌酶变性缓慢,在表面上变形过程呈现二态模型。
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Use of “phase diagram” of fluorescence to analyze the temperature dependence of unfolding intermediates of lysozyme
Abstract:The “phase diagram” of fluorescence was used to detect the intermediate of lysozyme unfolding and describe the unfolding pathway. At 4 oC, unfolding intermediate of lysozyme could be visible, which is consistent with three-state model. For the higher temperature disturbance, the intermediate was unfolded slowly and the unfolding process seems to be a two-state mechanism at 25 oC and 37 oC. At 4 oC, the intermediate was visible in presence of 5 M urea. However, the unfolding process was seemed to be a simple transition (Native state → Unfolding state) in presence of 4 M or 6 M urea, as the rate constants of the two transitions are different.
Keywords: Urea Lysozyme Unfolding model “phase diagram” of fluorescence
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No.3668011640125810****
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