神经干细胞移植促进脊髓损伤后BDNF基因的表达
首发时间:2009-12-28
摘要:目的:探讨神经干细胞(NSCs)移植对大鼠脊髓损伤后脑源性神经营养因子(BDNF)表达的影响及其意义。方法:NSCs提取自新生Wistar大鼠的海马区,经培养、鉴定。制作大鼠脊髓损伤(SCI)模型,于伤后第7天移植NSCs,实验分为3组:NSCs移植组(A组)、DMEM填充组(B组)、正常对照组(C组)。应用RT-PCR法和免疫组化法观察细胞移植后BDNF基因表达的变化。结果:RT-PCR结果分析,移植术后第1,3,5天,A组BDNF mRNA的表达量明显高于B组,差别有显著性意义(P<0.05)。组化结果分析,移植术后第7,14,28天BDNF的表达量明显高于B组,差别有显著性意义(P<0.05)。结论:NSCs在移植后可上调脑源性神经营养因子(BDNF)基因的表达,是其修复脊髓损伤的机制之一。
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Neural stem cells transplantation promote the expressions of brain derived neurotrophic factor after the spinal cord injury of rats
Abstract:Objective: To observe the effects of neural stem cells (NSCs) transplantation on the brain derived neurotrophic factor (BDNF) after the spinal cord injury (SCI) of rats,and to investigate the mechanism of repairing the SCI by NSCs transplantation. Methods: Neural stem cells were cultured from the hippocampus of rats’ embryo and identified by immuno- cytochemistry. Seven days after the operation of SCI, we transplanted the NSCs into the injured site. Sixty adult Wistar rats were randomly divided into three groups: SCI cured with NSCs transplantation (group A), SCI received DMEM solution (group B), control group (group C). Then the expression of BDNF of the lesion and neighbor areas were examined by Reverse transcsription polymerase chain reaction (RT-PCR) and immunohistochemistry,so as to investigated the mechanism of repairing the SCI after NSCS transplantation. RESULTS:Compared with group B, transplantation of NSCs enhanced the expression of BDNF mRNA than group A on the 1st, 3rd, 5th day after cell transplantation, and enhanced the expression of BDNF on the 7th, 14th, 28th day similarly. CONCLUSION:The transplantation of NSCs can change the tiny-entironment by upregulate the expression of BDNF. It is one of the mechanisms of repairing the SCI by NSCs transplantation. It maybe one of the mechanism of repairing the SCI by NSCs transplantation.
Keywords: Spinal cord injury Neural stem cells brain -derived neurotrophic factor
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