激光加工微通道用于微流动溶液的荧光检测的研究
首发时间:2010-01-07
摘要:本文介绍了一种在微米的微通道中静止的溶液和微流动的溶液的荧光检测的新方法,研究在微米级的微通道中溶液的微流动给荧光检测所带来的影响。实验结果表明:检测溶液在微米通道中单向流动,其接收到的荧光的强度与静止的溶液发出的荧光强度有所不同,两者相比较而言,单向流动的溶液发出的荧光波动幅度要大一些,且荧光强度整体增幅要推后。基于此研究可以广泛应用于在微尺度下生物分析和生物检测的研究与应用。
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Study on Fluorescent Detection of Microflowing Solution Using Laser-Processing Quartz Micro Channels
Abstract:A novel methods which based on fluorescent detection of immovable and microflowing solution is introduced in micron channels. Moreover effecting of fluorescent detection is induced by micro flowing solution in micron channels, which has been researched in the paper. The experimental results show that receiving fluorescence intensity has been varied in microflowing solution comparing with immovable solution because of unidirectional flow of detecting solution in micro channels. At the same time, fluctuating ranges of receiving fluorescence intensity has been increased, and the whole amplification of receiving fluorescence intensity is laggard in time domain. The new technology based on the study can be applied extensively in bioanalysis and biological detection in micro-scale application.
Keywords: Micro channel Laser-processing fluorescent detection quartz crystals
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