Apatite-wollastonite/Chitosan Composite Porous Scaffold Materials Research

• 1、College of Materials Science and Engineering, Sichuan University
• 2、Department of orthopedics of Jinzhou Medical Colleg

Abstract：AW bioactive glass ceramic have a good biological activity and biocompatibility, as well as the good mechanical properties. In this study, AW nano-precursor powder was prepared by sol - gel method, and AW / CS composite scaffold has been prepared by using organic foam impregnation process combined with physics package. Microstructure and mechanical properties of the scaffold material were tested by using scanning electron microscopy and compressive strength test instrument. In vitro cell compatibility of materials was evaluated by culturing t he rabbit bone marrow mesenchymal stem cells (bMSCs) together with the scaffold material. Tissue engineering of bone was built by the cells and the scaffold material in vitro and was implanted in rabbit bone defect. Biocompatibility and osteogenic capacity were evaluated by using the method of histological assessment of stent materials. The results showed that: composite scaffolds have the advantages of with large pore / pore structure, uniform pore distribution and interconnection. The large hole diameter is between the 100-500μm, and the porosity is about of 80% -90%. The composite scaffold is suitable for bone marrow stromal stem cells (MSC) adhesion, proliferation and differentiation, and is non-toxicity. Composite materials of animal cells in vivo studies have shown that new type of in vitro tissue engineering by compound with MSCs and AW / CS composite scaffold materials has a high osteogenic capacity and a good bone defect repair capacity.

Keywords： apatite-wollastonite chitosan scaffold bone marrow mesenchymal stem cells bone tissue engineering