Evaluating the nephrotoxicity of cyclosporin A in vitro

• 1、Department of Chemical and Biological Engineering, Zhejiang University

Abstract：During the drug development, the application of cell culture model for drug toxicity screening could facilitate to eliminate drug candidates with high toxicity, thus shorten the development phase and reduce the cost. To date, the monolayer culture model is the most commonly used in vitro model. But the toxicity of drugs in vivo could not always be accurately reflected. Therefore, this study employed the gel entrapment model for renal tubular epithelial cell culture. The results showed that the activities of alkaline phosphatase and multidrug resistant protein 2 (Mrp2) were better maintained in gel entrapment model, compared to monolayer culture. On the other hand, cyclosporin A was employed as a model drug to evaluate the feasibility of gel entrapped renal tubular epithelial cells in drug nephrotoxicity screening. Comapred with the toxic response and oxidative stress in monolayer culture model, gel entrapment culture model could sensitively reflect the nephrotoxicity induced by cyclosporin A in vivo.

Keywords： biological engineering nephrotoxicity gel entrapment culture monolayer culture cyclosporine A