Polymorphism study about Dipeptidyl Peptidase 10 in smokers with ChronicObstructive Pulmonary Disease in Chinese Han Population

• 1、Division of Pulmonary Disease, West China Hospital, Sichuan University

Abstract：To explore the Dipeptidyl Peptidase 10 (DPP10) gene polymorphism in smokers with chronic obstructive pulmonary disease (COPD) in Chinese Han population. Blood samples of 118 smokers with COPD and 143 health people were collected and their DNA samples were extracted by using Chelex-100. Then the gene types of the 11th exon linked to DPP10 were identified by a combination of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Use SPSS11.5 for windows to compare the mutation rates between the patients and the healthy.1)In the COPD group, there were 61 carriers of +591007 C/G of DPP10 GG genotype, 48 of GC genotype and 9 of CC genotype. In the control group, there were 67 carriers of GG genotype, 63 of GC genotype and 13 of CC genotype. The genotypes and allele frequencies in the COPD group were not significantly different from those in the control group (χ2＝0.647, P＝0.724; χ2＝0.616, P＝0.433, respectively). 2)Among the smokers with COPD, the genotypes and allele frequencies of SNP on +591007 C/G linked to DPP10 had no significant relationship with severity of disease (χ2＝2.914, P＝0.233; χ2＝1.560, P＝0.669, respectively).The gene polymorphism on +591007 C/G of DPP10 has no relationship with smokers with COPD.

Keywords： Chronic Obstructive Pulmonary Disease Dipeptidyl Peptidase 10 gene polymorphism