瘦素基因-2548 G /A多态性与慢性阻塞性肺疾病严重程度的关系
首发时间:2010-02-12
摘要:COPD是一种慢性全身多系统的炎症疾病,主要以不完全可逆的气道阻塞为特点。我们的目的是研究瘦素基因单核苷酸多态性(single nucleotide polymorphism, SNP;-2548 G/A)与COPD严重程度之间的关系。收集456例COPD患者和422例健康人血液样本,入组的COPD患者进行肺功能和体格检查根据GOLD I-IV级分级标准来分级,采用Chelex 100 分子生物纯树脂抽提全血DNA,运用限制性内切酶片段长度多态性方法检测瘦素基因-2548 G/A的基因型。统计分析该位点基因突变情况在COPD患者和健康人群中有无差异。我们发现瘦素基因-2548 G/A的基因型和等位基因频率在COPD患者和健康人群中的差异有统计学意义(OR =7.87; P = 0.000; OR = 2.98; P = 0.001; OR = 2.75; P = 0.000)。我们还发现瘦素瘦素基因-2548 G/A的多态性与COPD的严重程度相关(P < 0.05)。在这个病例对照研究中,我们发现在汉族人群中瘦素基因-2548G/A变异与COPD的易感性和严重程度相关。我们的结果显示瘦素2548位点可能是COPD严重程度的遗传学标记。
For information in English, please click here
The polymorphism -2548 G /A in leptin and COPD severity
Abstract:COPD is a chronic, multisystem, inflammatory disease characterized by airway obstruction that is not fully reversible. We aimed to investigate the role of -2548 G/A polymorphism of leptin gene in patients with COPD and its severity. A total of 456 subjects with COPD and 422 healthy controls were enrolled in this study in West China hospital. COPD patients have been undergone a spirometry and a physical examination by a chest physician to refer the GOLD I-IV stages. Analysis of -2548 G/A poly琺orphism of the leptin gene were performed by using the PCR-restriction fragment length polymorphism technique. The genotypes (GG, GA, and AA) and alleles (G and A) were scored and the frequency was estimated. The frequencies of the alleles and genotypes in patients and controls were compared. Both genotype and allele frequencies of leptin -2548 G/A were significantly different between COPD patients and controls(OR =7.87; P = 0.000; OR = 2.98; P = 0.001; OR = 2.75; P = 0.000). We also found significant relationship between leptin gene polymorphism and the severity of COPD (P < 0.05). In the present case-control study, we found an evidence of an association between the -2548 G/A variant of the leptin gene and COPD,disease severity among Chineses. Our results suggest that leptin 2548 could be used as genetic marker of COPD severity.
Keywords: Chronic Obstructive Pulmonary Disease leptin gene polymorphism
论文图表:
引用
No.4017851610612659****
同行评议
共计0人参与
勘误表
瘦素基因-2548 G /A多态性与慢性阻塞性肺疾病严重程度的关系
评论
全部评论0/1000