Selection of Microorganism with High Degradation Efficiency to Phenolic Acids From Soil of Continuous Cropping Poplar and Their Degradation

• 1、Forestry College of Shandong Agricultural University
• 2、Shandong Academy of Forestry

Abstract：One of the main reasons for replanted obstacles is the extensive accumulation of phenolic acids. Large area of managed poplar trees supports the wood demands in China. But long-term replanting of the same tree species lead to decline of plantation productivity. In this paper, Populous deltoids “I-69/55” was selected as the experimental material. And several common phenolic acids, ferulic acid, p-hydroxybenzoic acid, cinnamic acid and vanillin were selected as degradated targets. Microoganism which took this 4 phenolic acids as sole carbon source would be selected from the soil of replanted polpar plantation. 4 strains were selected and respectively identificated as Basidiomycete yeast sp., Aspergillus oryzae, Sinorhizobium sp. and Pseudomonas putida. The result of degradation experiments showed that: Degradation ability of 4 strains to ferulic acid were fairly good as the dagradation rates were all above 90% after 96 h’s treatments. Degradation rate of pseudomonas putida reached 91.1% at 48 h. Degradation rates of 4 strains to p-hydroxybenzoic acid were all above 90% after 96 h’s treatments. Degradation ability of Basidiomycete yeast sp. to cinnamic acid was higher as the degradation rate reached 98% after 72 h’s treatment. Degradation rate of pseudomonas putida to cinnamic acid was 88.2% after 96 h’s treatment, which was the lowest. Degradation ability of fungi (F2 and F3) to vanillin was higher than that of bacteria (B5 and B6). And all degradation courses of 4 strains to 4 phenolic acids could be well fitted by binary relations(R2>0.8).

Keywords： Continuous Cropping of Poplar Plantation Phenolic Acids Biodegradation