水稻基因PLDα3参与二化螟危害诱导的水稻挥发物的合成
首发时间:2010-04-08
摘要:磷脂酶D(Phospholipase D,PLD)是植物主要的磷脂水解酶,其产物磷脂酸是重要的信号调节物质,参与细胞的多种代谢途径以及植物对生物和非生物的逆境胁迫反应。然而,到目前为止,对于PLD是否参与植物抗虫反应,尤其是间接抗虫反应知之甚少。本文克隆鉴定了一个水稻PLD基因(PLDα3),并利用农杆菌转化法,获得了反义抑制PLDα3的2个单拷贝转基因水稻纯合品系。挥发物捕集结果表明,反义抑制品系与正常水稻健康苗的挥发物组成没有明显差异,但在受二化螟危害后,反义抑制品系挥发物总量较正常水稻的明显减少,两个品系分别为正常水稻的49.48%和57.94%;在单个挥发物组分方面,反义抑制品系有13种挥发物的释放量显著下降,2种挥发物的释放量明显上升。表明PLDα3参与二化螟危害诱导的水稻的间接防御反应。
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Rice gene PLDα3 is involved in the production of rice volatiles induced by the striped stem borer Chilo suppressalis (Walker)
Abstract:Phospholipase D (PLD) is a key enzyme in plants which can hydrolyze phospholipids to phosphatidic acid (PA), an important signal regulator in plants that participates in multi-metabolism pathways as well as plant stress responses. However, until now little is known about the role of PLDs in the herbivore-induecd plant defense responses, especially in the indirect plant defense responses. Here we cloned and identified a rice PLD gene PLDα3, and obtained two homozygous lines with antisense expression of PLDα3 and a single insertion by using an Agrobacterium-mediated transformation system. There was no significant difference in the volatiles emitted from non-manipulated plants of lines with antisense expression of PLDα3 and wild type (WT) lines. However, when plants were infested by rice striped stem borer (SSB) Chilo suppressalis, the total amount of volatiles emitted from plants of the two lines with antisense expression of PLDα3 was significantly lower than that from WT plants, which were 49.48% and 57.94% of that from WT plants, respectively. Moreover, compared to those from WT plants, levels of 13 chemicals from the two mutants decreased whereas 2 chemicals increased. This suggests an involvement of PLDα3 in SSB induced rice indirect defense responses.
Keywords: rice PLD Chilo suppressalis volatiles defense response
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