HPLC测定止咳药品中的柚皮苷含量
首发时间:2010-04-14
摘要:高效液相色谱(HPLC)是现代分析化学中最重要的分析方法之一。实验测定止咳药品中柚皮苷的含量。方法:采用高效液相色谱法。色谱柱:phenomenexC18柱(4.6mm×250mm,5μm);检测波长:283nm;流动相:甲醇-水-冰醋酸(38:62:1.5);流速:1.0ml/min;柱温:25℃。结果:柚皮苷线性范围为5-50μg/mL (r=0.9997)。结论:该方法简便快捷精密度高准确性好,可以作为环境药品的含量测定方法。同时,用数学统计方法对药品进行分析比较:苗族药咳速停糖浆柚皮苷含量与橘红颗粒柚皮苷含量无差别;其他两个药品中柚皮苷含量有极显著差异。
关键词: 高效液相色谱 环境药品 柚皮苷 含量测定 数学统计
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Determination of Naringin in medicine by HPLC
Abstract:High Performance Liquid Chromatography (HPLC) is one of the most important methods in modern chemical analysis. The content of Naringin in cough medicine was determined. Methods: the HPLC method was used. And the analytical column was C18 column, the UV detector wavelength was 283 nm. The mobile phase consist of methanol-water-glacial acetic acid (38:62:1.5), the flow rate was 1.0 ml/min, the column temperature was 25℃. Results: the calibration curve was linear in the range of 5-50μg/mL(r=0.9997). Conclusion: the method is simple、accurate、reliable and can be used for the quality control of Environmental medicine. At the same time, using mathematical statistical methods for analysis and comparison of the drugs,it was showed that the content of Naringin in the syrup drug of Miao Zu Ke Su Ting was the same as Ju Hong,and the contents of Naringin in the other two drugs show significant difference.
Keywords: HPLC Environmental medicine Naringin Determination Mathematics and Statistics
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No.4191445470812712****
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