基于二氧化硅纳米球免疫基底的表面增强拉曼散射液态免疫检测
首发时间:2010-09-08
摘要:报道了一种基于表面增强拉曼散射的简单、灵敏的液态免疫检测方法。该检测采用三明治免疫结构,其中利用固定有抗体的二氧化硅纳米球作为免疫基底,巯基苯甲酸标记的免疫金纳米颗粒作为免疫检测器。TEM结果表明免疫后,金纳米颗粒特异性吸附在二氧化硅纳米球表面,同时形成二聚及多聚体,产生许多SERS热点。SERS扫描结果证实,该方法是一种有效的基于SERS的液态免疫检测方法。
关键词: 表面增强拉曼散射(SERS) 免疫检测 二氧化硅 液态
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SERS-based aqueous immunoassay realized with immune silica nanoparticles
Abstract:A simple, sensitive SERS-based immunoassay realized in aqueous solution is demonstrated with a sandwich immune protocol. In such immunoassay, antibodies immobilized silica nanoparticles sever as the immune substrate while 4MBA-labeled immuno-Au nanoparticles are used as the immune sensors. According to the TEM images, it is clear that the immune gold nanoparticles are embedded onto the surfaces of the silica nanoparticles specifically after immunoreaction. As a result, the aggregations of gold nanoparticles have been formed with SERS-active "hot spots" on the dimer or multimer. The SERS scans confirm that the method proposed in this paper is an effective way for SERS-based aqueous immunoassay.
Keywords: Surface enhanced Raman scattering (SERS) immunoassay silica aqueous
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