浙贝精油化学成分和抑菌活性研究
首发时间:2010-10-22
摘要:目的:鉴定浙贝母精油的化学成分和抑菌活性。方法:水蒸汽蒸馏法制得精油,采用GC/MS仪自动分析结合保留指数分析化学成分;琼脂扩散法鉴定精油的抑菌活性。结果:分离得21种化学成分,可鉴定成分15种,占精油总离子流色谱峰面积的97.48%;对金黄色葡萄球菌和大肠杆菌均不敏感;对白色念珠菌抑菌圈直径为10.9mm。结论:水蒸气蒸馏浙贝精油主要成分为正十六酸(53.46%),(E,E)-9,12-十八烷二烯酸甲酯(26.96%),油酸(9.34%);对金黄色葡萄球菌和大肠杆菌无抑制效果,对白色念珠菌为中度敏感。
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Chemical composition and antimicrobial activities of essential oil from F.thunbergii Miq.
Abstract:The essential oil from dried bulbs of F. thunbergii Miq. obtained by hydrodistillation was analyzed by GC-MS combining with Kovats retention index. The results show that major constituents of this oil were n-Hexadecanoic acid (53.46%), (E, E)-9, 12-Octadecadienoic acid (26.96%) and Oleic Acid (9.34%) and 21 chemical components in the oil were separated and 15 chemical components were identified which represented 97.48% of the total peak. Our result showed that this oil have a moderate antifungal activity against Canidia albicans and no antimicrobial activity to Escherichia coli and Staphylococcus aureus at the concentration of 1×106 CFU/ml.
Keywords: F. thunbergii Miq Essential oil GC/MS Antimicrobial activity
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