Rapid and Sensitive Visual Detection of Methyl Parathion in Homogeneous Reaction Using Water Soluable Films with Immobilized Cholinesterase
首发时间:2010-10-27
Abstract:A rapid and sensitive visual detection of methyl parathion in homogeneous reaction was developed using water soluable poly(vinyl alcohol) (PVA) to immobilize cholinesterase (ChE). When used, a piece of ChE-PVA film is put into the solution and dissolved quickly. The released ChE from enzyme film is mixed and interacted fully with substrates and methyl parathion inhibitors. The color reaction is developed quickly in homogeneous system and the change or diference of color could be observed easily and clearly, while for common colorimetric sticks or strips for pesticides, it is dificult to discern the diference of developed color exactly due to the uneven dying on the surface of supporting matrix. With 10 min of inhibiton and 5 min enzyme reaction, 0.1 ?g/mL concentration of methyl parathion could be observed by visual detection.
keywords: Acetylcholinesterase Methyl Parathion Enzyme assay Immobilization PVA
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水溶性固定化胆碱酯酶酶膜在同相体系中对甲基对硫磷的快速敏感目视检测
摘要:本文介绍了一种快速准确测定甲基对硫磷的方法,使用水溶性聚乙烯醇固定胆碱酯酶。使用时,将一片胆碱酯酶酶膜置于溶液中迅速溶解。胆碱酯酶从酶膜中释放出来与底物以及甲基对硫磷抑制剂充分混匀反应。在同相体系中可以很快看到颜色变化,能够清楚地分辨研颜色的不同。而使用普通农残检测试纸条,由于在基质表面无法均匀染色,通常很难清楚地区分颜色的分别。抑制10分钟,反应5分钟,可以目视比色清楚分辨出0.1μg/mL。
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水溶性固定化胆碱酯酶酶膜在同相体系中对甲基对硫磷的快速敏感目视检测
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