小鼠睾丸特异性miR-471的表达及功能初步研究
首发时间:2010-11-26
摘要:miRNA在生物体的正常及病理过程中起重要的调控作用,然而miRNA在雄性生殖细胞中的功能目前仍所知甚少。本研究探讨了小鼠睾丸特异性miR-471在小鼠睾丸不同发育时期的表达水平,并利用已建立的小鼠精原干细胞体外培养系统初步研究其功能。过量表达miR-471的3T3稳定细胞株较之对照细胞株增殖更加活跃;类似地,在体外培养的小鼠精原干细胞中过量表达miR-471也能够促进精原干细胞的增殖。这一结果提示miR-471可能在小鼠睾丸发育中起促进增殖的作用。
关键词: 细胞生物学 miRNA 精原干细胞 过量表达 细胞增殖
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Preliminary Study of Mouse Testis-specific miR-471 expression and function
Abstract:miRNA plays important regulatory roles in various normal and malignant biological processes, however, its function in male germ cell development remains poorly elucidated. In this study we explorered temporal expression pattern of mouse testis-specific miR-471 in mouse testes. Furthermore, we investigated the function of miR-471 using established mouse spermatogonial stem cell in vitro transgenic model. We discovered that 3T3 stable cell line overexpressing miR-471 proliferate more actively than control cell line. Similarly, overexpressing miR-471 in cultured mouse spermatogonial stem cells also promotes cell proliferation. These results suggest that miR-471 may trigger cell proliferation during mouse testis development.
Keywords: cell biology miRNA spermatogonial stem cell overexpression cell proliferation
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