Core-shell nanoparticle SERS probe for Legionella pneumophilas detection

• 1、College of Environmental Science and Engineering, Hunan University

Abstract：It is of significant importance to rapidly detect and identify pathogen for diagnosis and treatment of infected disease. Surface-enhanced Raman Scattering (SERS) technology is of high sensitivity and can be used for single molecule detection. DNA probe based on metal nanoparticles are widely used in the area of medicine and biology. In this study, Ag/Au core-shell nanoparticle SERS gene probe was prepared and 4-mercaptobenzoic acid was used as Raman reporter. The core-shell nanoparticle SERS gene probe was used for the detection of mip gene of Legionella pneumophilas using sandwiched-type. The experimental results showed that obvious Raman signal can be observed in the presence of target probe using Ag/Au core-shell nanoparticle SERS as detection probe. But, no signal can be found in the absence of target probe. Therefore, gene probe labeled by Ag/Au core-shell nanoparticle SERS tags in this study provided a potential method for detection of Legionella pneumophilas.

Keywords： Surface-enhanced Raman scattering silver core gold shell nano-probes Legionella pneumophilas