聚乙烯中空纤维膜接枝固载甲酸脱氢酶的研究
首发时间:2010-12-22
摘要:采用紫外光照活化-接枝两步法,以二苯甲酮 (BP) 为光敏剂,将丙烯酸(AA)接枝到了聚乙烯(PE)中空纤维膜上,考察并确定了接枝条件,同时对改性后的膜做了扫描电子显微镜(SEM)表征。然后采用碳二亚胺盐酸盐(EDC)活化的方法将甲酸脱氢酶(FDH)固载到AA接枝的PE膜上,研究了固载条件对固载效率和催化性能的影响。结果表明,固载时间和EDC浓度对固载效率影响很大。当固载时间为24 h,EDC浓度为50 g/L时,固载效率接近100%, 固载量为426.7 mg酶/g载体。固载后的甲酸脱氢酶催化CO2还原甲酸收率可达 98.5%。
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Research on the immobilization of formate dehydrogenase on grafted PE hollow fibre membrane
Abstract:The PE hollow fibre membrane was modified by grafting acrylic acid(AA) with benzophenone(BP) as an initiator via UV irradiation activating-grafting two-steps method. The grafting conditions were examined and the optimum values were determined. Obtained membranes were characterized by SEM techniques. Formate dehydrogenase(FDH) was then linked to the film surface using EDC as an activator, and the effects of immobilization conditions on immobilization efficiency and catalytic performance were studied. It was found that both immobilization time and the concentration of EDC have important influences on immobilization efficiency, which is nearly 100%, and immobilization capacity is 426.7mg enzyme/g carrier in 24h with EDC concentration of 50 g/L. The yield of formic acid can reach 98.5% with membrane-attached FDH as catalysts for the reduction of CO2.
Keywords: PE membrane UV photo-grafting enzyme immobilization formate dehydrogenase
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No.4397970299829129****
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