蛋白质分子量对其结晶预混时传质过程的影响

阿萨德 艾哈迈德; 柴川; 吴江涛

0
0
浏览
下载

摘要
关键词
基金信息
论文图表
同行评议
相关论文
评论

Effect of protein molecular weight on the mass transfer in protein mixing

首发时间：2011-01-04

Asad Ahmed ¹
Asad Ahmed,Pakistan,(1975-),Male,PHD student,mainly research on molecular dynamic simulation,mass transfer and some other thermodynamics related field
Chai Chuan ¹ Wu Jiangtao ¹
Wu Jiangtao,(1975-),Male,Professor,mainly researched on new refrigerant,high accuracy measurement of thermoproperties and thermophysics field.

1、State Key Labratory of Multiphase Flow and Power Engineering, Xi\'an Jiaotong University

Abstract：Mixing of protein solution with that of precipitating agent is very important in the protein crystallization experiments. In this work, the interferometry images are recorded during the mixing of two proteins with different molecular weight: lysozyme of ~14.6 kDa and trypsin of ~ 23.3 kDa placed in a Mach-Zehnder interferometer. The protein molecular weight dependence on the competition of the transport process and kinetics at the interface are studied. The concentration profiles of protein solution are calculated to analyze the mass transfer during the mixing process. It is observed that the mass transfer process is more efficient during the mixing of protein with higher molecular weight. In addition, the more rapid concentration changes above the interface suggest that convection may dominate the diffusion. The phenomenon of convection is higher in the protein solutions with higher molecular weight.

keywords： Mass transfer Protein mixing Interface Diffusion Mach-Zehnder interferometer

点击查看论文中文信息

蛋白质分子量对其结晶预混时传质过程的影响

阿萨德艾哈迈德 ¹
Asad Ahmed,Pakistan,(1975-),Male,PHD student,mainly research on molecular dynamic simulation,mass transfer and some other thermodynamics related field
柴川 ¹ 吴江涛 ¹
Wu Jiangtao,(1975-),Male,Professor,mainly researched on new refrigerant,high accuracy measurement of thermoproperties and thermophysics field.

1、西安交通大学动力工程多相流国家重点实验室

摘要：蛋白质与沉淀剂溶液的预混过程在其结晶实验中尤为重要。搭建并应用Mach-Zehnder系统采集两种不同分子量的蛋白质分子（鸡蛋清溶菌酶~14.6kDa和牛胰蛋白酶~23.3kDa）结晶预混过程的干涉图像。对比研究了界面传质时物质输运过程与界面处流体的动力学特性。对反应场内的浓度场进行了计算用以分析预混过程中的传质过程。研究表明高分子量的蛋白质分子在预混过程中具有更高的传质效率。此外，界面以上的浓度变化较快也表明对流作用起主导作用，并且高分子量的蛋白质分子预混过程中的对流现象更加显著。

关键词：传质蛋白质结晶预混界面扩散 Mach-Zehnder干涉仪

基金：

论文图表：

引用

导出参考文献

.txt

.ris

.doc

Asad Ahmed,Chai Chuan,Wu Jiangtao. Effect of protein molecular weight on the mass transfer in protein mixing[EB/OL]. Beijing:Sciencepaper Online[2011-01-04]. https://www.paper.edu.cn/releasepaper/content/201101-100.

No.4401691561543129****

同行评议

共计0人参与

全部评论

0/1000

论文编号	201101-100
论文题目	蛋白质分子量对其结晶预混时传质过程的影响
文献类型
收录期刊	上传封面中文期刊英文期刊期刊名称（中文）期刊名称（英文）年，卷（）上传封面中文专著英文专著书名（中文）书名（英文）出版地出版社出版年上传封面中文译著英文译著书名（中文）书名（英文）出版地出版社出版年上传封面中文论文集英文论文集编者.论文集名称（中文） [c]. 出版地出版社出版年， - 编者.论文集名称（英文） [c]. 出版地出版社出版年，- 上传封面中文文献英文文献期刊名称（中文）期刊名称（英文）日期-- 在线地址http:// 上传封面中文文献英文文献文题（中文）文题（英文）出版地出版社,出版日期-- 上传封面中文文献英文文献文题（中文）文题（英文）出版地出版社,出版日期--
英文作者写法：中外文作者均姓前名后，姓大写，名的第一个字母大写，姓全称写出，名可只写第一个字母，其后不加实心圆点“.”, 作者之间用逗号“，”分隔，最后为实心圆点“.”, 示例1：原姓名写法：Albert Einstein,编入参考文献时写法：Einstein A. 示例2：原姓名写法：李时珍；编入参考文献时写法：LI S Z. 示例3：YELLAND R L,JONES S C,EASTON K S,et al.