Effects of Down-regulated miR-143 on Human Bronchial Epithelial Cells Exposed to Benzo[a]pyrene
首发时间:2011-01-14
Abstract:MicroRNAs(miRNAs) are small non-coding RNAs, which have the association with the occurrence or the development of the cancer. In this study, we investigated the effect of the down-regulated miR-143 on human bronchial epithelial cells (16-HBE) exposed to Benzo[a]pyrene [B(a)P]. Anti-miR-143 was transfected into cells to down-regulate the expression of miR-143. Cell proliferation, apoptosis and cycle of transfected cells were detected by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry after 24 h exposed of B(a)P, respectively. Our results confirmed that B(a)P had significant toxic effect on the cell proliferation, raised the apoptosis rate and blocked more cells at the S period in cell cycle (P<0.05). However, there was no significant difference between transfected group and non-transfected group after cells exposed to B(a)P (P>0.05). The results suggested that down-regulated miR-143 has no significant influence on cellar bio-effects induced by B(a)P under the present experimental conditions.
keywords: miR-143 B(a)P Human Bronchial Epithelial Cells Cell Proliferation Cell Apoptosis Cell Cycle
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Effects of Down-regulated miR-143 on Human Bronchial Epithelial Cells Exposed to Benzo[a]pyrene
摘要:MicroRNAs(miRNAs) are small non-coding RNAs, which have the association with the occurrence or the development of the cancer. In this study, we investigated the effect of the down-regulated miR-143 on human bronchial epithelial cells (16-HBE) exposed to Benzo[a]pyrene [B(a)P]. Anti-miR-143 was transfected into cells to down-regulate the expression of miR-143. Cell proliferation, apoptosis and cycle of transfected cells were detected by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry after 24 h exposed of B(a)P, respectively. Our results confirmed that B(a)P had significant toxic effect on the cell proliferation, raised the apoptosis rate and blocked more cells at the S period in cell cycle (P<0.05). However, there was no significant difference between transfected group and non-transfected group after cells exposed to B(a)P (P>0.05). The results suggested that down-regulated miR-143 has no significant influence on cellar bio-effects induced by B(a)P under the present experimental conditions.
关键词: miR-143; B(a)P; Human Bronchial Epithelial Cells; Cell Proliferation; Cell Apoptosis; Cell Cycle
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Effects of Down-regulated miR-143 on Human Bronchial Epithelial Cells Exposed to Benzo[a]pyrene
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