活细胞实时摄影测定微核的起源
首发时间:2011-02-18
摘要:微核被广泛应用于评估遗传损伤和染色体不稳定性。但受传统研究方法的限制,微核的起源并不清楚。近年来,长时间活细胞实时摄影和活细胞染色体荧光标记技术的发展使得在活细胞中实时追踪观察微核的起源成为可能。本文综述了应用该技术研究细胞中微核起源途径所得到的结果,同时讨论了该领域尚未解答的问题及可能的解决方案。
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Micronucleus formation detected by live-cell imaging
Abstract:Although micronuclei have been extensively used to evaluate genotoxic effects and chromosome instability, the most basic issue regarding their formation was not completely addressed until recently, due to limitations of traditional experimental methods. The development of live cell imaging, combined with genetically engineered chromosome labeling techniques makes it possible to investigate the origin of a micronucleus in a single cell in a real-time and high-throughput manner. Here we review all the available studies on the origins of micronuclei in live cells, and discuss novel findings based on this recently emerged methodology. Some unsolved questions on micronuclei formation and limitations of live cell imaging in the investigation of micronuclei have also been discussed.
Keywords: Live cell imaging Micronucleus
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No.4409822568333129****
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