Symbiotic Efficiency, Genetic Diversity and Phylogeny of the Rhizobia Isolated from Leucaena Leucocephala in Arid-hot River Valley Area in Panxi,Sichuan , China
首发时间:2011-03-28
Abstract:The symbiotic efficiency and diversity of the forty-two indigenous rhizobial isolates obtained from the root nodules of Leucaena leucocephala, one of the pioneer leguminous tree species of planted forests in arid-hot river valley area in Panxi, Sichuan, China, were characterized. The result of the inoculation test on their original host plants showed that the majority (86%) of the tested strains were ineffective. The genetic diversity of these rhizobia and the 17 reference strains were investigated by using PCR-RFLP of the 16S rRNA gene, 23S rRNA gene and the internal transcribed spacer (IGS) region between the 16S rRNA and 23S rRNA genes. The RFLP analyses and phylogenetic analyses based on the16S rRNA gene sequences of representative strains showed that Leucaena leucocephala hosted strains from four rhizobial genera, Sinorhizobium, Bradyrhizobium, Rhizobium and Mesorhizobium. One of the nodulating strains did not cluster with any of the reference strains. Phylogenetic analysis of 16S rRNA gene sequence of the strain indicated that the closest related genus was Herbaspirillum within the β-proteobacteria. This is the first record that Herbaspirillum of β-proteobacteria can be a specific symbiont of leguminous plant.
keywords: Leucaena leucocephala Rhizobia symbiotic efficiency phylogeny Arid-hot River Valley
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攀西干热河谷新银合欢根瘤菌的遗传多样性、系统发育及高效菌株的筛选
摘要:以分离自攀西干热河谷的42株新银合欢根瘤菌为研究对象,用水培共生结瘤试验研究其共生固氮有效性,用16S、IGS、23S rDNA PCR-RFLP研究其遗传多样性,用代表菌株的16S rRNA基因序列研究其系统发育。共生结瘤试验表明,有86%的新根合欢根瘤菌是无效菌株。6S、IGS、23S rDNA PCR-RFLP和16 S rRNA基因的系统发育表明,该区的新银合欢根瘤菌分属于Sinorhizobium、Bradyrhizobium、Rhizobium和 Mesorhizobium这四个属。但SCAU193例外,该菌株的16S rDNA序列表明,该菌株属于β-proteobacteria的Herbaspirillum属。这是这个属中能回接成功的根瘤菌的首次报道。
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No.4415719300451300****
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攀西干热河谷新银合欢根瘤菌的遗传多样性、系统发育及高效菌株的筛选
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