中江石泉丹参丛枝菌根真菌鉴定
首发时间:2011-03-17
摘要:目的:研究丹参根系是否有丛枝菌根真菌的侵染、侵染情况、丛枝菌根真菌的种类,以便通过接种丛枝菌根真菌来提高丹参的产量和质量。方法:采用碱解离-酸性品红染色法对根样进行染色处理,观察侵染情况并计算侵染率;采用湿筛倾析-蔗糖离心法从土样中分离丛枝菌根真菌的孢子,利用形态学分类特征鉴定其种属。结果:丛枝菌根真菌能侵染丹参根系并形成典型的丛枝菌根,侵染率为17.6%;从丹参根际土样中共分离出13种丛枝菌根真菌,其中球囊霉属(Glomus)9种,无梗囊霉属(Acaulospora)3种,盾巨孢囊霉属(Scutellspora)1种,鉴定到种的有8种,分别是Glomus caledonium、Glomus clarum、Glomus constrictum、Glomus geosporum、Glomus mosseae、Glomus sinuosum、Acaulospora scobiculata、Acaulospora lacunosa。其中尚有5种只鉴定到属。结论:丛枝菌根真菌是丹参丰产栽培中的一种潜在应用价值的生物资源。
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Identification of Arbuscular Mycorrhizal of Salvia miltiorrhiza Bunge in Shiquan of Zhongjiang
Abstract:Objective:To estimate colonization level、component of Arbuscular Mycorrhizal Fungi association of Salvia miltiorrhiza Bunge in Shiquan of Zhongjiang. Methods:Determine Arbuscular Mycorrhizal colonization level by alkaline lysising and acid fuchsin stain under light microscopy; Simultaneity, isolate spores of Arbusular Mycorrhizal Fungi with centriugation-sugar floatation from field-collected root-rhizosphere; Then identify the spores to genus basing on the morphological characters.Result:Arbuscular Mycorrhizal Fungi can infect the root of Salvia miltiorrhiza Bunge and form arbuscular mycorrhiza. And the colonization percentage is 17.6%. Specimen of spore or sporocarps of AMF was wet sieved from the collection of the rhizosphere soils, and 13 species of AMF were isolated from the field sample. Among these 13 species, 9 species belong to Glomus, 3 species bolong to Acaulospora, and only one belongs to Scutellospora. They were Glomus caledonium、Glomus clarum、Glomus constrictum、Glomus geosporum、Glomus mosseae、Glomus sinuosum、Acaulospora scobiculata and Acaulospora lacunosa. Additionaly, including 5 species only to category . Conclusion : Arbuscular mycorrhizal fungi may be a potent resource of biology which can stimulate the glowth of Salvia miltiorrhiza Bunge.
Keywords: Salvia miltiorrhiza Bunge Arbuscular mycorrhizal fungi Arbuscular mycorrhizae
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No.4414318566015129****
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