大豆苷原对去势大鼠子宫及乳腺的选择性作用及其ER和PPARγ表达的影响

邹世恩; 包蕾; 陈洁; 张绍芬

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大豆苷原对去势大鼠子宫及乳腺的选择性作用及其ER和PPARγ表达的影响

首发时间：2011-04-07

邹世恩 ¹
邹世恩, （1978-），男，主治医师，主要研究：围绝经医学、骨质疏松症、异位妊娠等发病机制及防治
包蕾 ² 陈洁 ³ 张绍芬 ²

1、复旦大学附属妇产科医院
2、复旦大学附属妇产科医院，上海市 200011
3、复旦大学附属产科医院，上海市 200011

摘要：目的：探讨大豆苷原对子宫内膜和乳腺组织的选择性作用及其机制。方法：将4月龄大鼠随机分为6组：假手术组（Sham）、去势组（OVX）、去势＋戊酸雌二醇组（E2，戊酸雌二醇800 μgokg-1od-1）、去势＋高剂量大豆苷原组（H-Dai，大豆苷原200 mgokg-1od-1）、去势＋中剂量大豆苷原组（M-Dai，大豆苷原50 mgokg-1od-1）和去势＋低剂量大豆苷原组（L-Dai，大豆苷原10 mgokg-1od-1）。除假手术组外，其余大鼠均切除卵巢，术后4周开始以相应的药物灌胃，3个月后处死大鼠。取大鼠子宫及乳腺组织做相应检测。结果：H-Dai组大鼠子宫内膜腔上皮、间质和内膜厚度显著厚于OVX组（P<0.01）和L-Dai组（P<0.05）。乳腺腺泡计数Sham组大鼠为9.1个，E2组为2.3个，其余组未见明显腺泡。H-Dai组大鼠子宫内膜ERα mRNA表达显著高于OVX组（P<0.01），且高于L-Dai组。乳腺组织ERα表达差异无统计学意义。ERβ在3个剂量Dai组大鼠子宫内膜和乳腺组织均未检测出，3个剂量Dai组之间PPARγ差异无统计学意义。结论：大豆苷原对去势大鼠子宫内膜和乳腺组织具有选择性作用。高剂量大豆苷原可能通过刺激子宫内膜ERα，对内膜产生弱雌激素样作用，中、低剂量则无刺激性作用。大豆苷原对乳腺组织则无刺激作用。

关键词：大豆苷原；子宫内膜；乳腺；受体雌激素；受体过氧化物酶增殖子激活

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The Tissue-selective Effect of Daidzein On Uterus and Lacteal Gland and the Expression of Estrogen Receptors and Peroxisome Proliferator-activated Receptor Gamma of the Ovariectomized Rats

ZOU Shien ¹
邹世恩, （1978-），男，主治医师，主要研究：围绝经医学、骨质疏松症、异位妊娠等发病机制及防治
BAO Lei ² CHEN Jie ² ZHANG Shaofen ²

1、Obstetrics and Gynecology Hospital of Fudan University
2、Obstetrics and Gynecology Hospital of Fudan University，Shanghai 200011

Abstract：Objective: To investigate the tissue-selective effect of daidzein on uterus and lacteal gland and it's molecular mechanisms. Materials and methods: Sixty four-month-old Sprague-Dawley（SD）female rats were allotted to one of six groups. Four weeks after reproducing the osteoporosis model of ovariectomized SD rats, Sham group and control OVX were administrated with distilled water, whereas the other groups received valerate estriol（800 μgokg-1od-1）and different doses of daidzein（10,50,200 mgokg-1od-1）by gavage respectively for three months. After the sacrifice of the rats, we detected histomorphometry of uterine endometrial and lacteal gland tissue, and the expressions of mRNA of ERα, ERβ and PPARγ realtime-RT-PCR, while protein were by immunohistochemistry in them. Results: Endometrial luminal epithelium, stroma, and endometrial thickness of rats in H-Dai group were significantly thicker than that of OVX group (P<0.01) and L-Dai group (P<0.05). Lacteal gland of rats in Sham group was counted as 9.1, while E2 group was 2.3, no clear gland in other groups. The expression of ERα mRNA of rats' endometrial in H-Dai group was significantly higher than that of OVX group (P<0.01), and higher than that of L-Dai group. The expression of ERα in breast tissue was no significant difference. The expression of ERβ rats' endometrial and breast tissue in three Dai groups were not detected, and the expression of PPARγ was not significant different among three Dai groups. Conclusion：There was tissue-selective effect of daidzein on uterus and lacteal gland of the ovariectomized rats. High-dose daidzein may play weak estrogen-like effect on uterine endometrial tissue through ERα, while no estrogen-like stimulation on breast.

Keywords： Daidzein Uterine endometrial tissue Lacteal gland Estrogen receptor Peroxisome proliferator-activated receptorγ

基金：

1. 教育部新教师基金资助项目（No. 20090071120020）

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邹世恩，包蕾，陈洁，等. 大豆苷原对去势大鼠子宫及乳腺的选择性作用及其ER和PPARγ表达的影响[EB/OL]. 北京：中国科技论文在线 [2011-04-07]. https://www.paper.edu.cn/releasepaper/content/201104-147.

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论文编号	201104-147
论文题目	大豆苷原对去势大鼠子宫及乳腺的选择性作用及其ER和PPARγ表达的影响
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