COPD大鼠模型海马区和血清中BDNF蛋白的表达变化
首发时间:2011-05-03
摘要:目的 探讨BDNF在COPD大鼠模型海马区和血清中的表达情况,以及了解被动吸烟和气管内注入脂多糖的干预是否参与此过程。方法 采用被动吸烟和气管中注入脂多糖建立大鼠COPD模型。将24只大鼠随机分为4组,每组各6只,分别为正常对照组,吸烟组,脂多糖组以及吸烟+脂多糖组(即COPD组)。造模28天后,应用酶联免疫吸附实验(ELISA)方法测定各组大鼠血清中BDNF的表达水平,再通过免疫组化染色和图像分析方法半定量检测大鼠海马区BDNF的表达情况。结果 COPD组、吸烟组和脂多糖组血清及海马区BDNF表达水平均较正常组明显升高(p<0.05),且COPD组血清及海马区BDNF表达水平均较吸烟组和脂多糖组明显降低(p<0.05)。结论 COPD大鼠海马区及血清中BDNF蛋白表达水平升高,且被动吸烟和气管内注入脂多糖均可诱导BDNF蛋白表达水平升高。
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The Expression of BDNF in Hippcocampal and Serum of COPD Model Rats
Abstract:AIM: In clinic,we have found that cognitive impairment frequently occurs in chronic obstructive pulmonary disease (COPD) patients,but little is known about its pathogeny. And, researchers confirmed that brain-derived neurotrophic factor (BDNF) may be involved in the pathophysiology of cognitive impairment and is affected by many factors, for example,smoking,infection,hypoperfusion, hypoxia, et al. In this study, we aimed to explore the effects of passive smoking and intratracheal instillation of lipopolysaccharide (LPS) ,alone or in combination,on BDNF expression in hippcocampal and serum . METHODS : In consideration of smoking and infection are two main inducement of COPD, we established the rat COPD model by passive smoking and intratracheal instillation of LPS. Four rat groups were studied,with equal age and gender ratios in each:control (n=6), smoking (n=6),LPS (n=6) and smoking+LPS (n=6,COPD model). Level of BDNF in serum was measured by ELISA.And the expression of BDNF in hippcocampal was assessed using immunohistochemistry and image analysis method. RESULTS :We found that compared to controls,BDNF in hippcocampal and serum significantly increased after smoking, LPS and smoking + LPS exposure. However,BDNF increased less evident in smoking + LPS model than in smoking or LPS model both in hippcocampal and serum . CONCLUSIONS :Upregulation of BDNF in hippcocampal and serum of COPD group.And smoking and intratracheal instillation of LPS (alone or in combination) exposure can increase BDNF level in hippcocampal and serum.
Keywords: COPD BDNF smoking LPS cognitive function
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