吡那地尔、美托洛尔、谷氨酰胺、胰岛素单独及联合使用对H9c2心肌细胞缺氧/复氧损伤的抗凋亡作用及机制
首发时间:2011-05-20
摘要:目的:研究吡那地尔(pinacidil, Pin)、 美托洛尔(metoprolol, Met)、谷氨酰胺(glutamine, Glu)、胰岛素(insulin, Ins)四药联用对缺氧/复氧(hypoxia/reoxygenation, H/R)所致H9c2心肌细胞损伤的抗凋亡保护作用并探讨其作用机制。方法:将培养的H9c2心肌细胞随机分为7组,即(1) 正常对照(Con)组;(2) 缺氧/复氧(H/R)组;(3) 吡那地尔(Pin)组;(4) 美托洛尔(Met)组;(5) 谷氨酰胺(Glu)组;(6) 胰岛素(Ins)组;(7) 吡那地尔、美托洛尔、谷氨酰胺和胰岛素四药联用(PMGI)组。细胞经药物处理建立缺氧复氧损伤模型,并测定各组H9c2心肌细胞的存活率;收集培养液测定乳酸脱氢酶(lactate dehydrogenase, LDH)活性;流式细胞仪检测心肌细胞凋亡百分率;罗丹明123 (Rhodamine123, Rh123)荧光探针标记线粒体,检测荧光强度以反映心肌细胞线粒体膜电位(mitochondrial membrane potential, △Ψm)变化。结果:对于缺氧/复氧损伤的H9c2心肌细胞,吡那地尔、美托洛尔、谷氨酰胺与胰岛素四药联用组与药物单用组或H/R组相比能明显提高细胞存活率,保护其细胞膜,减少LDH渗漏;减少细胞凋亡率;提高△Ψm. 结论:吡那地尔、美托洛尔、谷氨酰胺和胰岛素四药联用后,通过不同机制保护心肌细胞线粒体,增强了细胞抗凋亡作用。
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Antiapoptosis Effects And Mechanism Of Pinacidil, Metoprolol, Glutamine, Insulin Single And Combination On H9c2 Cardiac Myocytes Exposed To Simulated Hypoxia/Reoxygenation
Abstract:Objective: To investigate protective effects and mechanism of pinacidil, metoprolol, glutamine, insulin on H9c2 cardiac myocytes exposed to simulated hypoxia/reoxygenation .Methods: H9c2 cardiac myocytes were randomly divided into seven groups: (1) Control group: cardiomyocytes were seeded in normoxic incubation; (2) Hypoxia/reoxygenation (H/R) group; (3) Pinacidil (Pin) group; (4) Metoprolol (Met) group; (5) Glutamine (Glu) group; (6) Insulin (Ins) group; (7) Pinacidil + Metoprolol + Glutamine + Insulin (PMGI) group. The survival rates of cardiomyocytes were detected. The activity of lactate dehydrogenase (LDH) in the culture medium were measured. The mitochondria were labeled by Rhodamine123 fluorescence probe and the fluorescent intensity produced by Rh123 was measured, which reflects changes of mitochondrial membrane potential (MMP, △Ψm). The apoptosis rates were measured with flow cytometry. Results: The combination use of pinacidil, metoprolol, glutamine, insulin can protect the H9c2 cardiac cell membrane caused by hypoxia/reoxygenation injury; increase cell survival rate, decrease LDH leakage, decrease the apoptosis rate and increase the mitochondrial membrane potential. Conclusion: Compared to the individual drug, the combination using the different of the mechanisms protects the mitochondria of cardiac myocyte ,decreases the injury and enhances antiapoptosis effection of myocardial cells.
Keywords: Hypoxia/Reoxygenation Lactate dehydrogenase antiapoptosis Mitochondrial membrane potential
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吡那地尔、美托洛尔、谷氨酰胺、胰岛素单独及联合使用对H9c2心肌细胞缺氧/复氧损伤的抗凋亡作用及机制
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