文昌鱼(Branchiostoma japonicum) AmphiH2A-core和AmphiH2A.X基因的克隆及其表达分析
首发时间:2011-07-12
摘要:性腺发育是一个复杂的多基因参与调控的过程,目前针对该领域的研究主要集中于高等的脊椎动物。文昌鱼是现存生物中与脊椎动物最为相似的无脊椎动物,其器官构造和基因组结构简单,是研究脊椎动物器官及基因家族起源的新型模式生物。因此了解文昌鱼的性腺发育机理,不仅有助于加快文昌鱼的模式化进程,还将为了解脊椎动物性腺发育机理的起源提供帮助。为此,我们在前期工作中构建了文昌鱼(Branchiostoma japonicum)性腺差减文库,并获得了一系列精巢、卵巢特异表达的候选基因。本文对其中一个卵巢特异表达基因AmphiH2A.X及其同源基因AmphiH2A-core的进化和表达进行了系统分析,发现两者在基因结构和编码蛋白的一级序列都与其对应的脊椎动物同源基因高度相似,如AmphiH2A.X含有哺乳动物H2A.X的典型标志性结构--SQEY功能域;与脊椎动物相同,文昌鱼AmphiH2A-core也属于广谱型表达基因,但与脊椎动物不同的是AmphiH2A.X特异表达于文昌鱼的卵巢中,且其表达量随着卵巢成熟度的提高而不断增长。由此可以推断H2A-core在文昌鱼及脊椎动物中具有相似的功能,而H2A.X在文昌鱼中的主要功能可能是参与其卵巢的发育。
关键词: 文昌鱼 AmphiH2A.X AmphiH2A-core 卵巢发育
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Sequence identification and expression pattern of AmphiH2A-core and AmphiH2A.X genes in amphioxus Branchiostoma japonicum
Abstract:Many genes and signaling pathways are involved in the process of animal gonad development. Nevertheless, our current knowledge about the mechanism of this process is limited to vertebrates mainly. Amphioxus is the closest living invertebrate relative of the vertebrates, with simple body plan and genome content, and is an emerging model organism for elucidating the complexities of vertebrate organs and gene families. Thus studies on the mechanism of amphioxus gonad development is not only shedding certain insights on the origin of the mechanism of vertebrate gonad development, but also promoting amphioxus modeling process. To this end, we previously constructed subtracted cDNA libraries for amphioxus gonads and identified a set of genes which are potentially involved in amphioxus gonad development. In this study, we focused on one of these ovary-specific genes AmphiH2A.X and its related genes AmphiH2A-core and found that they have similar gene structures and also encode similar proteins sequences with their vertebrate cognates. For example, AmphiH2A.X protein contains a canonical SQEY structure, the feature found in other H2A.X. In regard to expression pattern, AmphiH2A-core expresses in all examined organs from the adult, the pattern similar to vertebrate H2A genes, however the expression of AmphiH2A.X is mainly detected in ovary and increased as the ovary preceding to be mature. Therefore it could be concluded that AmphiH2A-core play conserved roles in both vertebrates, while amphioxus H2A.X have evolved a specific function in amphioxus ovary development
Keywords: amphioxus AmphiH2A.X AmphiH2A-core ovary development
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文昌鱼(Branchiostoma japonicum) AmphiH2A-core和AmphiH2A.X基因的克隆及其表达分析
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