Regular patterns and characteristics of adult neurogenesis in Phrynocephalus przewalskii

• 1、School of Life Science, Lanzhou University
• 2、School of Life Science, Lanzhou University, Lanzhou 730000

Abstract：[Objective] To investigate the specific pattern of adult neurogenesis in Phrynocephalus przewalskii -- typical reptile in northwest of China. [Methods] Lizards were intraperitoneally injected with BrdU in order to label dividing cells. Distinct immunocytochemistry were performed: DAB-immunostaining and fluorescence-immunostaining. Confocal microscope imaging was used to observe the location and cell production. Golgi staining technology was carried out in order to indentify the morphology structure of neural cells in medial cortex of the lizard. [Results] Compared with mammal, the cytoarchitectonical patterns of Phrynocephalus przewalskii own simpler characteristics without typical hippocampal formation. BrdU-labeled newborn cells are mostly distributed in the ventricular zone of lateral ventricle with greater production of cells in region referred to anterior olfactory nucleus. A large quantity of GFAP labeled radial glial cells are arranged in the telencephalon, absent of free astroglia. Besides, the major cell type in medial cortex is projection neuron which shows various morphology structures, with collateral branches extending bilateral plexiform layers or gathered in cluster protrudent to dorsal cortex. [Conclusion] The distribution and proliferation rate of newborn adult neural cells in telencephalon of Phrynocephalus przewalskii lizard living in natural conditions will provide fundamental basis to study the effect of different stimuli and exposure on the adult neurogenesis of reptiles.

Keywords： neurobiology neurogenesis cell proliferation immunocytochemistry Phrynocephalus przewalskii