Study on the Cytotoxicity of Gold Nanoparticle Colloids

• 1、High School Attached to Capital Normal University
• 2、School of Material Sciences and Engineering, Beijing Institute of Fashion Technology, Beijing 100029

Abstract：In this paper，we aim to study the cytotoxicity of gold nanoparticle colloids. We studied the effect of gold nanoparticle colloids of diameter 15nm to 20nm with sodium citrate solution and those with the mixture of sodium citrate and Poly Vingl Pyrrolidone (PVP) solution to the activity of normal cells of Human skin(human epidermal cell, Human hypertrophic scar derived fibroblasts also called HSF) and cancer cells(HeLa, K562, CHO) by 3H-TdR incorporation method. Results demonstrate that gold nano-particle colloids within a certain concentration range could promote the proliferation of normal cells of Human Skin in a time-dependent and dose-dependent manner. If the concentration was below 50μmol / L, the facilitation of gold nanoparticle colloids towards cell proliferation strengthened as the concentration increased. At 50μmol / L, the facilitation ability was most significant. However, the facilitation ability decreased as the concentration continually increased above 50μmol / L, and gradually converted to inhibitive effect. The activity of cancer cells showed invisible changes when the concentration of Gold nano-particle colloids was less than 50μmol / L, and when the concentration was above 50μmol / L, the inhibitive effect on cancer cell increased as the concentration increased.

Keywords： Cell Biochemistry Gold nanoparticle colloids 3H-TdR incorporation method Cytotoxicity