the effect of homocysteine on the function of granulosa cells

• 1、peking university,reproductive center of peoples hospital,beijing(100044)
• 2、peking university,the central laboratory of peoples hospital,beijing(100044)

Abstract：Objective: To study the effect of homocysteine on function of granulosa cells in vitro. Methods: The granulosa cells cultured in vitro was used and homocysteine was added in culture medium. By the method Western Blot we observed the effect of homocysteine on activation of MAPK, By ELASA the level of progesterone secreted by granulosa cells was measured.To determine the impact of homocysteine on environmental stability in cells, the level of glutathione was also detected.To make sure if the interaction between homocysteine and folic acid was existed, all the experiments were repeated by granulosa cells cocultured with folic acid with or without homocysteine, the data were compared and analyzed. Results: HCY can significantly activate the activity of p-ERK1 / 2. But the effect was not dependented on the concentration. When treated with 50μM, HCY can significantly promote progesterone secretion by granulosa cells. The follic acid can not change the activation of MAPK by homocysteine, but can significantly inhibited the level of progesterone even if homocysteine was exist.The level of glutathione in granulosa cells was reduced when coculture with either the follic acid or homocysteine. Conclusion: It is explicit that high level of homocysteine has extensive impact on the function of granulosa cells. The follic acid may play a regulatory role during the progesterone secretion stimulated by homocysteine, but it still can not reverse the decline of intracellular glutathione levels by homocysteine.

Keywords： oocyte development homocysteine folli acid granulosa cell MAPK