Altered expression of plant miRNAs and their target mRNAs in tomato flower and fruit upon virus infections
首发时间:2012-01-05
Abstract:The economic importance of tomato is well documented, and tomato has become a model for fleshy fruit development and ripening studies. Plant microRNAs (miRNAs) are small endogenous RNAs that are involved in a variety of activities, including plant development, signal transduction and protein degradation, as well as response to environment stress, and pathogen invasion. Here in this study, we aimed at quantifying the expression alterations of nine miRNAs and target mRNAs in tomato flower and fruit development upon CMV and TAV infections. Three different Cucumber mosaic virus (CMV) strains CMV-Fny, CMV-Fny2b, CMV-Fny-satT1 and Tomato aspermy virus (TAV)-Bj were used in our investigation, and the miRNA/mRNA expression alterations were analyzed by real-time quantitative RT-PCR. The results shown the levels of several miRNA/mRNA pairs were increased upon virus infection. However, the increased levels of individual miRNAs differed for different virus strains, reflecting differences in severity of symptom phenotypes. The altered expression pattern of these miRNA/mRNA pairs and their predicted functions indicate the possible roles in flower and fruit development, and provide experimental data for understanding the miRNA-mediated phenotype alterations in tomato fruit.
keywords: Solanum lycopersicum Cucumber mosaic virus Tomato aspermy virus tomato microRNA gene expressionord
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病毒侵染对寄主番茄花和果子microRNAs及其靶标mRNAs表达的影响
摘要:番茄不仅具有重要的经济价值,并且番茄已成为研究肉质果子成熟和发育的模式植物。microRNA(miRNAs)是一类小的内源RNAs,它参与植物体的各种生理活性,包括植物发育、信号传导、蛋白质降解、环境胁迫应答及病毒防御等,本研究旨在应用实时荧光定量RT-PCR的方法,检测在三种黄瓜花叶病毒(Cucumber mosaic virus ,CMV)株系 CMV-Fny, CMV-Fny2b, CMV-Fny-satT1和番茄不孕病毒(Tomato aspermy virus ,TAV)的侵染下,寄主番茄花和果子中,九对miRNAs/mRNAs表达量的差异。结果表明,有几对miRNAs/mRNAs在病毒的侵染下有明显的上调,然而,miRNAs在不同的病毒侵染下,其表达量上调水平不一,并表现出不同的症状表型。这些miRNAs/mRNAs表达量的变化及对miRNAs/mRNAs功能的预测,指示了它们在植物花和果子发育中的潜在作用,为理解miRNA介导的花和果子表型变化提供了实验参考依据。
关键词: 番茄 黄瓜花叶病毒 番茄不孕病毒 番茄microRNA 基因表达
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