Obstructive jaundice nuclear receptor FXR exercise intervention with expression study in the rats

• 1、Institute of Physical Education Hunan Normal University, Changsha 410081

Abstract：Objective: Through the small or medium strength aerobic exercise intervention, observed 7 days and 14 days of obstructive jaundice rats to mild liver injury FXR mRNA expressions. Methods: 36 SD rats were divided into obstructive jaundice, early exercise and later exercise interventions 3 groups, exercise group speed by 5%, and the slope of 12.0 m/min, thirty minutes a day, trained 14 days of small or medium strength training run sets. Exercise end rats after fasting 12 hours, and then separating out the liver tissue and materials, HE staining of liver cell morphology change; biochemical analysis methods of serum total bilirubin (TBIL)，glutamic-pyruvic transaminase (ALT) and glutamic-oxaloacetic transaminase (AST) contents; RT-PCR detection of FXR and β-actin mRNA expression levels. Result: HE staining of liver cells in the obstructive jaundice group showed extensive vacuolar degeneration of liver cells, necrosis, early exercise group showed a small amount of spotty necrosis of liver cells, later exercise group not have obvious cell necrosis; serum levels examination showed early exercise group and later exercise group SD serum TBIL, AST and ALT content than the obstructive jaundice liver damage group content is low （P<0.05）; RT-PCR assay techniques showed early exercise group and later exercise group compared with SD rats EGF mRNA expression level was higher obstructive jaundice（P<0.05）. Conclusion : Small or medium strength aerobic exercise for resistance to damage the liver of obstructive jaundice have a good effects, its mechanism may enhanced FXR expression and promoted the bowel bile acid enterohepatic circulation and excretion, play a role of liver repair itself.

Keywords： Obstructive jaundice The small or medium strength aerobic exercise Farnesoid X Receptor SD rats