The study of neural stem cells survival and differentiation on surface modified PHBHHx film

• 1、School of life science and technology, Xi'an Jiaotong University, Xi'an 710049
• 2、Medical School, Xi'an Jiaotong University, Xi'an 710049

Abstract：Objective: In order to enhance the biocompatibility of PHBHHx films with neural stem cells (NSCs), we modified the surface of PHBHHx films, and then to lay the foundation for application of NSCs-based brain tissue engineering. Method: PHBHHx film was fabricated by a solution-casting method, and the morphology of the lm was observed under scanning electron microscopy(SEM); The film was treated by NaOH or lipase, then the surface hydrophilic property was characterized by water contact angle measurement; NSCs were isolated from the cerebral cortex of rat embryos on embryonic day 14-15, and P3 cells were seeded on surface treated PHBHHx films, after that the distribution of NSCs on films were studied by immunostaining, morphology of cells attach on the film were observed under SEM, Immune cell fluorescence chemistry was used to study the differentiation of NSCs. Result: Water contact angle of films treated with NaOH or lipase decreased dramatically compared with untreated; NSCs could survive on surface treated films, compare with untreated films the number of NSCs was increased, NSCs could also differentiate into neurons and glia cells on these films. Conclusion: The amelioration of hydrophilic property of PHBHHx film can improve its biocompatibility with NSCs.

Keywords： PHBHHx neural stem cells surface treatment