Direct electrochemistry and electrocatalysis of hemoglobin modified with quantum dots and immobilized in chitosan film on glassy carbon electrode
首发时间:2012-02-07
Abstract:Hemoglobin (Hb) modified with mercaptopropionic acid-stabilized CdTe quantum dots (QDs) was immobilized on a glassy carbon electrode (GCE) by chitosan (CS). QDs could accelerate the electron transfer between Hb and the electrode, as well as its catalytic ability toward nitrite (NO2). The modified electrode displayed a couple of stable and well-defined redox peaks in aqueous-ethanol mixtures. UV-Vis spectroscopy analysis showed that the modified Hb could keep its natural structure. The modified electrode showed an electrocatalytic activity to the reduction of NO2 without the aid of an electron mediator. The electrocatalytic response showed a linear dependence on the NO2 concentration ranging from 7.5 to 120.0 μM with a fairly low detection limit of 2.5 μM (S/N = 3).
keywords: Hemoglobin Quantum dots Chitosan Direct electrochemistry Electrocatalysis
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固载于壳聚糖膜内的量子点促进的血红蛋白的直接电化学及电催化
摘要:本文利用壳聚糖(CS)将CdTe量子点(QDs)修饰的血红蛋白(Hb)有效地固载到玻碳电极(GCE)表面,制备了GCE /Hb-QDs/CS薄膜修饰电极。QDs不仅能够促进Hb与电极间的直接电子转移,而且提高了该修饰电极对亚硝酸根的催化性能。紫外-可见光谱分析结果表明QDs修饰的Hb仍然能够保持其良好的天然结构。在缓冲液与乙醇体积比为1的混合溶液中,固载于电极表面的Hb呈现出一对稳定的、准可逆氧化还原峰。所制备的修饰电极对亚硝酸根的还原具有良好的电催化响应,线性响应范围为7.5-120.0μmol/L,检出限为2.5μmol/L。
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