应用于荧光检测的硅藻阵列化芯片
首发时间:2012-02-20
摘要:硅藻壳体完全由二氧化硅构成,并拥有复杂的三维微纳孔结构,可以被应用在生物芯片以及微流体器件领域。本文提出了一种将硅藻图案化排列并且连接在玻璃基片表面的方法。热熔胶EVA以及光刻技术被用于排列及连接硅藻。EVA的加热温度和时间被调整到60 oC和2分钟,来保证硅藻具有最好的定位精度和连接强度。扫描电镜及能谱分析仪被用来分析阵列化连接后的硅藻表面。当荧光蛋白被滴加到复合基片表面后,测得带有阵列化硅藻的EVA基片表面荧光蛋白的荧光强度,是空白EVA基片表面荧光蛋白的荧光强度的2.5倍。因此,该复合基片大幅提高了荧光检测的灵敏度。
关键词: 机械设计 硅藻 生物芯片 EVA热熔胶 光刻 阵列
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Micro-arraying of diatom microshells on glass substrate for fluorescence spectroscopy application
Abstract:With delicate there-demensional porous structures and SiO2 texture, diatom can be used in the fields of biosensors and microfludic devices. In this paper, a fabrication technique for patterning and bonding diatoms with the glass substrate has been developed to expand the application of diatoms. Hot-melt ethylene-vinyl acetate copolymer (EVA) and photoetching techniques are used to bond and patteren diatoms. The patterning and bonding conditions are adjusted by varying reheating time and temperature of EVA. And diatom array can be formed with high positioning accuracy and bonding quality when EVA is reheated at 60 oC for 2 min. The surface of diatoms is analyzed after the bonding and patterning process with SEM and EDS. In the end, fluoresceine isothiocyanate (FITC) is added over the compound substrate. The fluorescence intensity of FITC is 2.5 times higher than that of FITC over pure glass and 1.4 times higher than that of FITC over dispersed diatoms. This compound diatom array substrate can highly improve the fluorescence detecting sensitivity.
Keywords: mechanical layout diatoms biosensor EVA photoetching array
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