基于微流控芯片的固定化葡萄糖氧化酶催化反应动力学及热力学的探究
首发时间:2012-03-13
摘要:本文提出了一种基于微流控芯片来研究固定化酶(Glucose Oxidase, GOD)的催化动力学及热力学参数的方法,因为无需考虑传统的量热法和微量量热法必须使用的绝热系统。本方法利用Arrhenius方程来建立固定化酶催化反应的动力学和热力学参数之间的关系。计算得到的固定化GOD催化反应活化能(Ea=22.45 kJ/mol)以及活化焓 (ΔHa= 19.94 kJ/mol)比自由酶小,表明固定化的GOD比自由状态的酶热稳定性更好。本工作提出的这种获取酶催化反应的动力学以及热力学参数的方法,必将促进我们对固定化酶催化反应的理解。
关键词: 分析化学 固定化酶 动力学参数 热力学参数 温度控制 Arrhenius方程
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Exploring the Temperature-Dependent Kinetics and Thermodynamics of Immobilized Glucose Oxidase in Microchip
Abstract:Herein, we report a method to investigate the thermodynamics and kinetics of immobilized enzyme (Glucose Oxidase, GOD) catalytic reaction on a microfluidics platform with precise temperature-control using a home-made plexiglass temperature controllable holder. This approach allows us to extract kinetic and thermodynamic parameters of the immobilized enzyme catalytic reactions easily without involving the thermally isolated system, showing significant advantages over the traditional calorimetric and microcalorimetric methods which require complex fabrications of thermally isolated system. In our approach, Arrhenius equation was introduced to establish the relationship between the kinetic and thermodynamic parameters of the immobilized GOD. Results show that the obtained activation energy (Ea=22.45 kJ/mol) and the activation enthalpy (ΔHa= 19.94 kJ/mol) are smaller than free enzymes, demonstrating that the immobilized GOD exhibits improved thermal stability compared with free enzymes. The present work offers an alternative approach to achieve the kinetics and thermodynamics of immobilized enzyme catalytic reactions on a microfluidics chip and will certainly promote our understanding of enzyme catalytic reactions.
Keywords: Analytical chemistry Immobilized GOD Kinetic parameters Kinetic parameters Temperature control Arrhenius equation
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基于微流控芯片的固定化葡萄糖氧化酶催化反应动力学及热力学的探究
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